Transcriptome and metabolome analyses reveal the regulation of peel coloration in green, red Chinese prickly ash (Zanthoxylum L.)

Abstract

Peel colour is an important external economic characteristic of Chinese prickly ash cultivars (Zanthoxylum bungeanum Maxim.). To gain insight into their coloration mechanisms, we performed an integrated analysis of green and red peels using combined metabolomic and transcriptomic analyses. Pelargonin-O-hexoside-O-rhamnoside-O-hexoside, pelargonidin 3,5-diglucoside, peonidin O-hexoside, cyanidin O-syringic acid and peonidin 3-O-glucoside were found to be the key anthocyanins. Transcriptome data indicated that the anthocyanidin synthase genes and UDP-glucose flavonoid 3-O-glucosytransferase genes were significantly increased to promote the redness of the peels. In addition, we discussed the role of R2R3-MYB transcription factors in coloration, of which the c80935 and c226097 genes may be the key regulatory factors for anthocyanin biosynthesis. Generally, this is the first study to identify and reveal the main anthocyanins in Chinese prickly ash peels during different developmental periods. The results of this research lay the foundation for understanding the regulation of coloration in Chinese prickly ash peels.