Transcriptome and expression profile analysis of highly resistant and susceptible banana roots challenged with Fusarium oxysporum f. sp. cubense tropical race 4.

Ting-Ting Bai,Zi-Lin Wu,Xiao-Lei Ruan,Wan-Bin Xie,Hua-Ping Li,Ping-Ping Zhou,Jie Sun,Ling Zhou,Wen-Chao Xiao,Zhengguang Zhang

doi:10.1371/journal.pone.0073945

Ting-Ting Bai, Zi-Lin Wu + Show 8 more

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https://doi.org/10.1371/journal.pone.0073945

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Journal: PloS one	Publication Date: Sep 23, 2013
Citations: 77	License type: CC BY 4.0

Affiliation: South China Agricultural University

Abstract

Banana wilt disease, caused by the fungal pathogen Fusarium oxysporum f. sp. cubense 4 (Foc4), is regarded as one of the most devastating diseases worldwide. Cavendish cultivar ‘Yueyoukang 1’ was shown to have significantly lower disease severity and incidence compared with susceptible cultivar ‘Brazilian’ in greenhouse and field trials. De novo sequencing technology was previously performed to investigate defense mechanism in middle resistant ‘Nongke No 1’ banana, but not in highly resistant cultivar ‘Yueyoukang 1’. To gain more insights into the resistance mechanism in banana against Foc4, Illumina Solexa sequencing technology was utilized to perform transcriptome sequencing of ‘Yueyoukang 1’ and ‘Brazilian’ and characterize gene expression profile changes in the both two cultivars at days 0.5, 1, 3, 5 and 10 after infection with Foc4. The results showed that more massive transcriptional reprogramming occurs due to Foc4 treatment in ‘Yueyoukang 1’ than ‘Brazilian’, especially at the first three time points, which suggested that ‘Yueyoukang 1’ had much faster defense response against Foc4 infection than ‘Brazilian’. Expression patterns of genes involved in ‘Plant-pathogen interaction’ and ‘Plant hormone signal transduction’ pathways were analyzed and compared between the two cultivars. Defense genes associated with CEBiP, BAK1, NB-LRR proteins, PR proteins, transcription factor and cell wall lignification were expressed stronger in ‘Yueyoukang 1’ than ‘Brazilian’, indicating that these genes play important roles in banana against Foc4 infection. However, genes related to hypersensitive reaction (HR) and senescence were up-regulated in ‘Brazilian’ but down-regulated in ‘Yueyoukang 1’, which suggested that HR and senescence may contribute to Foc4 infection. In addition, the resistance mechanism in highly resistant ‘Yueyoukang 1’ was found to differ from that in middle resistant ‘Nongke No 1’ banana. These results explain the resistance in the highly resistant cultivar and provide more insights in understanding the compatible and incompatible interactions between banana and Foc4.

Highlights

Musa spp. is one of the most important fruit crops in the world and their global annual production account to more than 120 Mt [1]
Our study revealed that Chitin elicitorbinding protein (CEBiP) was significantly induced in ‘Yueyoukang 1’ at the first four time points, while it was only induced in ‘Brazilian’ at 5 and 10 days post infection (DPI), which is in agreement with the report that the expression of CEBiP were up-regulated in middle resistant ‘Nongke No 1’ compared to ‘Brazilian’ 2 and 4 days after tropical race 4 (TR4) infection [15]
No literature reported that Brassinsteroid insensitive 1-associated receptor kinase 1 (BAK1) was involved in PAMPtriggered immunity (PTI) triggered by fungus, our results revealed that the number of differentially expressed genes (DEGs) related to BAK1 was the most extensive in all kinds of the pattern-recognition receptors (PRRs)

Summary

Introduction

Musa spp. (bananas and plantains) is one of the most important fruit crops in the world and their global annual production account to more than 120 Mt [1]. (bananas and plantains) is one of the most important fruit crops in the world and their global annual production account to more than 120 Mt [1]. Musa crop has the prominence as a dessert fruit, and provides a vital source of food to many inhabitants of the humid tropics. Like that of all other crop species, is affected by distinct constraints, among which Fusarium wilt caused by Fusarium oxysporum f. The disease had seriously affected banana cultivation for more than 60 years in tropical America of the last century. It was under control only when the susceptible cultivar Gros Michel was replaced by the resistant Cavendish banana cultivars [3]. Foc, a new race of Foc, is found to be able to infect Cavendish cultivars and has caused great damage to Cavendish production worldwide in recent years [2]

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