Abstract
Stems and roots of Kadsura plant species were the significant ingredients of traditional Chinese medicine. Kadsura heteroclita is one of the popular medicinal plants used in Tujia and Yao nationalities of China. Antioxidant compounds like lignan, sesquiterpenoid and triterpenoid are the major active components of K. hetroclita. Mass cultivation and bio-manufacturing strategies were being proposed to meet the increasing demand of Kadsura species plant parts. Therefore, it is important to reveal the molecular networks involved in biosynthesis of these highly efficient medicinal compounds. Here, transcriptomes of roots, stems and leaves in K. heteroclite seedling were sequenced by Hiseq2000 and unigenes involved in biosynthesis of lignan, sesquiterpenoid and triterpenoid biosynthesis were mined. As a result, 472 million clean reads were obtained which after aligning resulted in 160,248 transcripts and 98,005 genes. 191 and 279 unigenes were expected to be involved in biosynthesis of lignan, sesquiterpenoid and triterpenoid biosynthetic pathways respectively. Lignan, sesquiterpenoid and triterpenoid biosynthesis pathway genes were highly significant and differentially upregulated in roots and stems and downregulated in leaves. Also, genes encoding for MYB and bHLH transcription factors possibly involved in regulation of lignan, sesquiterpenoid and triterpenoid biosynthesis were discovered. These results provide the fundamental genomic resources for dissecting of biosynthetic pathways of the active components in K. hetroclita.
Highlights
Kadsura heteroclita (Roxb.) Craib, popularly known in China as jixueteng, dixuexiang, sanxuexiang, dazuangufeng, chuifengsan, dafengshateng, dahongzuan, is a medicinal plant belonging to genus Kadsura and Received: 18 Aug 2020
Kadsura heteroclita is popularly known for its medicinal importance owing to its key ingredients used in traditional Chinese medicine (TCM) in Tujia and Yao Nationality
K. heteroclita has been used for centuries, the cellular and molecular by mechanisms involved in regulating the biosynthesis of the medicinal components, especially lignan, sesquiterpenoid and triterpenoid, is not known till date
Summary
Kadsura heteroclita (Roxb.) Craib, popularly known in China as jixueteng, dixuexiang, sanxuexiang, dazuangufeng, chuifengsan, dafengshateng, dahongzuan, is a medicinal plant belonging to genus Kadsura and Received: 18 Aug 2020. Not Bot Horti Agrobo 48(4):1802-1831 family Schisandraceae. It is mainly distributed in Hubei, Guangdong, Hainan, Guangxi, Guizhou, Yunnan provinces of China, Bangladesh, Vietnam, Laos, Myanmar, Thailand, India, Sri Lanka, etc. Rattan and root parts of K. heteroclita exhibit several medicinal properties such as promoting Qi, relieving pain, expelling wind and dehumidification, which is mainly used to treat rheumatism and rheumatoid arthritis, low back pain, lumbar muscle strain, stomach pain, abdominal pain, hemiplegia, postpartum paralysis, dysmenorrhea, fracture and bruise (Editorial Committee of Flora of China, 1996; Zhang et al, 2019; Cao et al, 2020). Modern studies have reported that lignans, sesquiterpenes and triterpenes are main components of rattan and root in K. heteroclita. The lignans, sesquiterpenes and triterpenes exhibit various pharmacological functions such as anti-oxidation, anti-virus, anti-tumor, cardiovascular protection, liver protection, anti-inflammatory, analgesic, and in memory improvement respectively (Zhang et al, 2019)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
