Transcriptome analysis reveals the molecular mechanism of boron deficiency tolerance in leaves of boron-efficient Beta vulgaris seedlings

Abstract

Sugar beet (Beta vulgaris L.) has a high demand for B, and B deficiency inhibits normal growth and productivity. However, there is a lack of information on how B deficiency affects the growth of beet at the transcriptome level, and the factors that govern B utilisation efficiency. This study aimed to identify the genes differentially expressed under B deficiency and those that underlie the mechanisms of efficient B use in two sugar beet cultivars. Accordingly, B-efficient (H, KWS1197) and B-inefficient (L, KWS0143) sugar beet cultivars were used, and two levels of boron were employed in the hydroponic experiments: B0.1 (0.1 μM B, deficiency) and B50 (50 μM B, CK). The results showed that B deficiency inhibited leaf growth, significantly reduced B concentration and B transfer coefficient, and increased peroxidase (POD) activity and malondialdehyde and proline content. The transcriptome data showed that the B-efficient variety exhibited more differentially expressed genes than the B-inefficient variety. Metabolic pathways were the most critical pathways involved in the B deficiency response. The expression of POD, bHLH, WRKY transcription factors, and nodulin26-like intrinsic protein (NIP5;1) were upregulated in the KWS1197 variety. In conclusion, the KWS1197 variety had physiological advantages and a highly efficient B utilisation molecular mechanism, contributing to a high B deficiency tolerance. This study provides a theoretical basis for the adaptation mechanism to B deficiency in sugar beets.