Abstract
Hibiscus hamabo Siebold & Zuccarini (H. hamabo) is tolerant to salt and drought conditions, but the molecular mechanisms that underlie this stress tolerance remain unclear. In this study, the transcriptome of H. hamabo roots was investigated under NaCl or PEG treatment. A total of 20,513 and 27,516 significantly changed known genes at 6 h and 24 h, respectively, were detected between the salt or drought treatments and the control libraries. Among these, there were 3845 and 7430 overlapping genes under the two stresses at 6 h and 24 h, respectively. Based on the analysis of enriched KEGG pathways and clustering of expression patterns, the DEGs that were continuously up- or down-regulated under both salt and drought treatments were mainly enriched in MAPK signaling pathway, transcription factors, transporters and other pathways. The transcriptome expression profiles of H. hamabo provide a genetic resource for identifying common regulatory factors involved in responses to different abiotic stresses. In addition, the identified factors may be useful to developing genetic breeding strategies for the Malvaceae.
Highlights
Water-deficit and salinity are abiotic stresses that severely impact various cellular and whole plant processes, influencing plant yield and quality [1]
The clean reads were aligned to the reference genome sequence, and the results showed that the total mapped ratio of all samples was higher than 64.00%, indicating that there was a little contamination of phycophyta from hydroponic environment, the mapped ratio was relatively high, which can be used for subsequent analysis (Table 2)
To understand how H. hamabo genes respond to salt or drought stress, we first compared the mRNA populations with Principal component analysis (PCA) (Figure 1a)
Summary
Water-deficit and salinity are abiotic stresses that severely impact various cellular and whole plant processes, influencing plant yield and quality [1]. These stress conditions can reduce the availability of water for basic cellular functions and affect the maintenance of turgor pressure [2], while salinity can cause an ionic imbalance [3]. MPK6 could activate MYB41 to response to salt stress in Arabidopsis [7]. The MYB transcription factor family is widely distributed in plants and has been reported playing roles in regulating plant-specific biological processes, as responsing and creasing tolerance to biotic stresses [9]. There may be differences in the adaptive mechanisms used by different plants under salt and drought stresses, and these require further exploration, especially in non-model plants
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