Abstract
Circulating microRNAs presented in venous plasma have been demonstrated as powerful biomarkers for the complex diseases like cancer. Nevertheless, those presented in arterial plasma remained largely unexplored. Here, using microarray technique, we compared microRNA expression profiles of the matched arterial and venous plasma samples from the same male rats. Though the microRNA profiles were largely similar, we identified 24 differentially expressed microRNAs, including 10 arterial highly expressed microRNAs and 14 venous highly expressed microRNAs. The differentially expressed microRNAs were validated by qRT-PCR. Computational analysis of these microRNAs and their targets indicated that arterial highly expressed microRNAs were overrepresented for functional terms like hematopoiesis and diseases like Crohn's Disease and leukemia; while venous highly expressed microRNAs were enriched for cell differentiation function, and diseases like distal myopathies and heart failure. Our analysis also suggested significant correlations between plasma microRNA expression and tissue microRNA expression. Four arterial highly expressed microRNAs also showed enriched expression in specific tissues and would be novel biomarker candidates.
Highlights
MicroRNAs, as the core component of the posttranscriptional regulation machinery, play important roles in many biological processes like cell proliferation, differentiation, stress response and apoptosis [1]
Circulating microRNAs presented in venous plasma have been demonstrated as powerful biomarkers for the complex diseases like cancer
By comparing the venous plasma microRNA expression profiles from patients with Takotsubo cardiomyopathy or acute myocardial infarction, miR-16 and miR-26a were found to be highly expressed in Takotsubo cardiomyopathy patients, while miR-1 and miR133a were highly expressed in acute myocardial infarction patients
Summary
MicroRNAs, as the core component of the posttranscriptional regulation machinery, play important roles in many biological processes like cell proliferation, differentiation, stress response and apoptosis [1]. By comparing the venous plasma microRNA expression profiles from patients with Takotsubo cardiomyopathy or acute myocardial infarction, miR-16 and miR-26a were found to be highly expressed in Takotsubo cardiomyopathy patients, while miR-1 and miR133a were highly expressed in acute myocardial infarction patients. These microRNAs, when used in combination, were proven to be an accurate biomarker for discriminating these clinically indistinguishable, life threatening diseases [9]. By comprehensive profiling of blood microRNAs from 454 individuals, the deregulated circulating microRNAs across 14 disease situations (including but not limited to lung cancer, pancreatic ductal adenocarcinoma, melanoma) were screened and demonstrated [7]
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