Transcriptome analysis reveals interplay between hormones, ROS metabolism and cell wall biosynthesis for drought-induced root growth in wheat

Abstract

The ability of roots to grow under drought stress is an adaptive trait for crop plants especially under rain fed and restricted irrigation regime. To unravel the molecular mechanism of drought induced-root growth, root transcriptomes of two wheat genotypes viz. Raj3765 and HD2329, with contrasting root growth under drought stress were analyzed. Drought stress significantly enhanced total root length in Raj3765 as compared to that of HD2329. RNA-seq analysis led to the identification of 2783 and 2638 differentially expressed genes (DEGs) in Raj3765 and HD2329, respectively, under drought stress as compared with non-stress conditions. Functional annotation, gene ontology and MapMan analysis of the DEGs revealed differential regulation of genes for pathways associated with root growth and stress tolerance. Drought stress significantly upregulated auxin receptor (AFB2) and ABA responsive transcription factors (MYB78, WRKY18 and GBF3) in roots of Raj3765. Although certain genes for ethylene pathway were downregulated in both the genotypes, ACC oxidase and 2OG-Fe(II) oxygenase were upregulated only in Raj3765 which might contribute to maintenance of a basal ethylene level to maintain root growth. Several genes related to cell wall biosynthesis and ROS metabolism were significantly upregulated in Raj3765. Genes related to gibberellic acid, jasmonic acid and phenylpropanoid pathways were downregulated in roots of both the genotypes under drought stress. Our analysis suggests that a coordinated yet complex interplay between hormones, cellular tolerance, cell wall synthesis and ROS metabolism are required for drought induced root growth in wheat.