Abstract
BackgroundFusarium wilt, caused by Fusarium oxysporum f. sp. cucumerinum (Foc), is a severe disease affecting cucumber (Cucumis sativus L.) production worldwide, but mechanisms underlying Fusarium wilt resistance in cucumber remain unknown. To better understand of the defense mechanisms elicited in response to Foc inoculation, RNA sequencing-based transcriptomic profiling of responses of the Fusarium wilt-resistant cucumber line ‘Rijiecheng’ at 0, 24, 48, 96, and 192 h after Foc inoculation was performed.ResultsWe identified 4116 genes that were differentially expressed between 0 h and other time points after inoculation. All ethylene-related and pathogenesis-related genes from the differentially expressed genes were filtered out. Real-time PCR analysis showed that ethylene-related genes were induced in response to Foc infection. Importantly, after Foc infection and exogenous application of ethephon, a donor of ethylene, the ethylene-related genes were highly expressed. In response to exogenous ethephon treatment in conjunction with Foc inoculation, the infection resistance of cucumber seedlings was enhanced and endogenous ethylene biosynthesis increased dramatically.ConclusionCollectively, ethylene signaling pathways play a positive role in regulating the defense response of cucumber to Foc infection. The results provide insight into the cucumber Fusarium wilt defense mechanisms and provide valuable information for breeding new cucumber cultivars with enhanced Fusarium wilt tolerance.
Highlights
Fusarium wilt, caused by Fusarium oxysporum f. sp. cucumerinum (Foc), is a severe disease affecting cucumber (Cucumis sativus L.) production worldwide, but mechanisms underlying Fusarium wilt resistance in cucumber remain unknown
Fusarium wilt (FW) is a typical soil-borne disease caused by Fusarium oxysporum f. sp. cucumerinum (Foc) [1], which usually results in severe losses in global cucumber output [2]
Quality analysis of RNA sequencing (RNA-seq) data from cucumber roots infected by Foc Roots of cucumber ‘Rijiecheng’, the Foc-resistant line, were sampled with three biological replicates and sequenced for transcriptome analysis
Summary
Fusarium wilt, caused by Fusarium oxysporum f. sp. cucumerinum (Foc), is a severe disease affecting cucumber (Cucumis sativus L.) production worldwide, but mechanisms underlying Fusarium wilt resistance in cucumber remain unknown. Cucumerinum (Foc), is a severe disease affecting cucumber (Cucumis sativus L.) production worldwide, but mechanisms underlying Fusarium wilt resistance in cucumber remain unknown. The defense mechanisms induced in a plant upon exposure to a pathogen, including the network of cross-communicating signaling pathways, may halt pathogen infection. The predominant symptom elicited by Foc infection of cucumber is wilting, which is caused by disturbance of the water balance [19, 20]. It is known that ET signaling pathways play a crucial role in the inducible plant defense response [18, 25]. Liu et al [27] observed that ET signaling pathways might positively regulate the defense response of Panax notoginseng to Fusarium solani
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