Abstract
Iron is a trace metal that is found in animals, plants, and the human body. Human iron absorption is hampered by plant iron shortage, which leads to anemia. Leafy vegetables are one of the most direct and efficient sources of iron for humans. Despite the fact that ferrotrophic disorder is common in calcareous soil, however, non-heading Chinese cabbage performs a series of reactions in response to iron deficiency stress that help to preserve iron homeostasis in vivo. In this study, we discovered that iron deficiency stress caused leaf yellowing and impeded plant development in both iron-deficient and control treatments by viewing or measuring phenotypic, chlorophyll content, and Fe2+ content in both iron-deficient and control treatments. We found a total of 9213 differentially expressed genes (DEGs) in non-heading Chinese cabbage by comparing root and leaf transcriptome data with iron deficiency and control treatments. For instance, 1927 DEGs co-expressed in root and leaf, including 897 up-regulated and 1030 down-regulated genes, respectively. We selected some key antioxidant genes, hormone signal transduction, iron absorption and transport, chlorophyll metabolism, and transcription factors involved in the regulation of iron deficiency stress utilizing GO enrichment, KEGG enrichment, multiple types of functional annotation, and Weighted Gene Co-expression Network Analysis (WGCNA). This study identifies prospective genes for maintaining iron homeostasis under iron-deficient stress, offering a theoretical foundation for further research into the molecular mechanisms of greater adaptation to iron-deficient stress, and perhaps guiding the development of iron-tolerant varieties.
Highlights
Iron is a trace metal that plays a role in a variety of physiological and metabolic processes, making it one of the most important trace elements for human health
This study provides a theoretical foundation for further unraveling the molecular mechanism of important genes in nonheading Chinese cabbage iron homeostasis regulation, as well as some guidance for future non-heading Chinese cabbage varieties with high iron content
The hydroponic experiment revealed that on the fourth day of hydroponics, there was no significant difference in plant height between the two treatments [iron deficiency treatment: 0 mol/L Fe2+; control: 0.02 mol/L Fe2+], but the leaves under iron deficiency had a slight green loss compared to the control leaves (Figure 1A)
Summary
Iron is a trace metal that plays a role in a variety of physiological and metabolic processes, making it one of the most important trace elements for human health. As the major component of hemoglobin, iron’s most significant physiological role is to assist in oxygen intake, transport, and release (Zong et al, 2021). Iron is a necessary trace element in plants and plays a vital role in their vegetative growth (Zhang et al, 2021). Photosynthesis, respiration, chlorophyll biosynthesis, nitrogen sulfur assimilation, and hormone biosynthesis are just a few of the fundamental cellular activities where iron plays a significant role (Briat et al, 2015; Connorton et al, 2017). Plants have evolved extensive adaptive mechanisms to maintain intracellular iron homeostasis in response to Fe2+ depletion through physiological, metabolic, and gene regulation over lengthy periods in response to Fe2+ deficit (Li and Lan, 2017)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
