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Abstract
The vetch (Vicia sativa) is one of the most important annual forage legumes globally due to its multiple uses and high nutritional content. Despite these agronomical benefits, many drawbacks, including cyano-alanine toxin, has reduced the agronomic value of vetch varieties. Here, we used 454 technology to sequence the two V. sativa subspecies (ssp. sativa and ssp. nigra) to enrich functional information and genetic marker resources for the vetch research community. A total of 86,532 and 47,103 reads produced 35,202 and 18,808 unigenes with average lengths of 735 and 601 bp for V. sativa sativa and V. sativa nigra, respectively. Gene Ontology annotations and the cluster of orthologous gene classes were used to annotate the function of the Vicia transcriptomes. The Vicia transcriptome sequences were then mined for simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers. About 13% and 3% of the Vicia unigenes contained the putative SSR and SNP sequences, respectively. Among those SSRs, 100 were chosen for the validation and the polymorphism test using the Vicia germplasm set. Thus, our approach takes advantage of the utility of transcriptomic data to expedite a vetch breeding program.
Highlights
The legume family (Fabaceae) is the third-largest family of flowering plants and the second most important plant family in agriculture [1]
Raw data from the 454 sequencing run was submitted to the National Center for Biotechnology
A total of 42,405 of the sativa sequence reads were fully incorporated into the assembly, resulting in 2698 contigs or isotigs along with 34,938 singletons (Table 1B)
Summary
The legume family (Fabaceae) is the third-largest family of flowering plants and the second most important plant family in agriculture [1]. “Green manuring” refers to incorporation of the soil of any field or forage crop while the crops are green or soon after flowering [4] This procedure adds nutrients and organic matter to the soil, which prevents soil erosion, and helps suppress weeds, insect pests and disease, as well. Known as the common vetch (hereafter, vetch), is one of the most commonly-grown winter cover crops [6]. It is used as pasture, silage and hay. Due to its economic and ecological advantages, vetch is widespread throughout many parts of world, including the Mediterranean Basin, west and central Asia, China, eastern Asia, India and the USA [6,7]
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