Transcriptome Analysis of Two Species of Jute in Response to Polyethylene Glycol (PEG)- induced Drought Stress

Zemao Yang,Ruike Lu,Qing Tang,Jianguang Su,Bibo Wu,Zhigang Dai,Ying Xu,Chaohua Cheng

doi:10.1038/s41598-017-16812-5

Zemao Yang, Ruike Lu + Show 6 more

Open Access

https://doi.org/10.1038/s41598-017-16812-5

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Abstract

Drought stress results in significant crop yield losses. Comparative transcriptome analysis between tolerant and sensitive species can provide insights into drought tolerance mechanisms in jute. We present a comprehensive study on drought tolerance in two jute species—a drought tolerant species (Corchorus olitorius L., GF) and a drought sensitive species (Corchorus capsularis L., YY). In total, 45,831 non-redundant unigenes with average sequence length of 1421 bp were identified. Higher numbers of differentially expressed genes (DEGs) were discovered in YY (794) than in GF (39), implying that YY was relatively more vulnerable or hyper-responsive to drought stress at the molecular level; the two main pathways, phenylpropanoid biosynthesis and peroxisome pathway, significantly involved in scavenging of reactive oxygen species (ROS) and 14 unigenes in the two pathways presented a significant differential expression in response to increase of superoxide. Our classification analysis showed that 1769 transcription factors can be grouped into 81 families and 948 protein kinases (PKs) into 122 families. In YY, we identified 34 TF DEGs from and 23 PK DEGs, including 19 receptor-like kinases (RLKs). Most of these RLKs were downregulated during drought stress, implying their role as negative regulators of the drought tolerance mechanism in jute.

Highlights

As well, many plant metabolism pathways participate in response to abiotic stress, such as photosynthetic, phenylalanine metabolism, peroxidase, MAPK signalling pathways, etc
We carried out transcriptome sequencing in C. capsularis and C. olitorius exposed to drought stress to explore the drought resistance mechanism in these cultivated species
Drought is one of the major environmental factors affecting the geographical distribution of plants in nature

Summary

Introduction

Many plant metabolism pathways participate in response to abiotic stress, such as photosynthetic, phenylalanine metabolism, peroxidase, MAPK signalling pathways, etc. Acid[10], sinapic acid[11], isocitrate dehydrogenase (ICDH), superoxide dismutase (SOD), catalase (CAT)[7], etc If these pathways are able to reconstruct the new balance and preserve ROS at a relativly low level, the tissues and cells may be prevented from damage and death because of oxidation. Jute (Corchorus spp.) is the second most important global biodegradable natural fibre crop It is a diploid annual crop (2n = 14) distributed in the tropics, subtropics, and warm temperate regions of the world (mainly in Asia and Africa)[14]. It includes two cultivated species, C. capsularis L., a drought sensitive species, and C. olitorius L., a drought tolerant species. We carried out transcriptome sequencing in C. capsularis and C. olitorius exposed to drought stress to explore the drought resistance mechanism in these cultivated species

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