Transcriptome analysis of two bloom-forming Prorocentrum species reveals physiological changes related to light and temperature

Abstract

Temperature and light substantially influence red tide succession. However, it remains unclear whether the molecular mechanisms differ among species. In this study, we measured the variation in the physiological parameters of growth and pigments and transcriptional levels of two bloom-forming dinoflagellates, namely Prorocentrum micans and P. cordatum. This was undertaken in four treatments that represented two factorial temperature combinations (LT: 20 °C, HT: 28 °C) and light conditions (LL: 50 µmol photons m-2 s-1, HL: 400 µmol photons m-2 s-1) for 7-day batch culture. Growth under high temperature and high light (HTHL) was the fastest, while growth under high temperature and low light (HTLL) was the slowest. The pigments (chlorophyll a and carotenoids) decreased significantly in all high light (HL) treatments, but not in high temperature (HT) treatments. HL alleviated the low light-caused photolimitation and enhanced the growth of both species at low temperatures. However, HT inhibited the growth of both species by inducing oxidative stress under low light conditions. HL mitigated the HT-induced stress on growth in both species by upregulating photosynthesis, antioxidase activity, protein folding, and degradation. The cells of P. micans were more sensitive to HT and HL than those of P. cordatum. This study deepens our understanding of the species-specific mechanism of dinoflagellates at the transcriptomic level, adapting to the future ocean changes including higher solar radiation and higher temperatures in the upper mixed layer.