Abstract
Leaf spot disease caused by the fungus Fusarium proliferatum (Matsushima) Nirenberg is a destructive disease of tomato plants in China. Typical symptoms of infected tomato plants are softened and wilted stems and leaves, leading to the eventual death of the entire plant. In this study, we resorted to transcriptional profile analysis to gain insight into the repertoire of effectors involved in F. proliferatum–tomato interactions. A total of 61,544,598 clean reads were de novo assembled to provide a F. proliferatum reference transcriptome. From these, 75,044 unigenes were obtained, with 19.46% of the unigenes being assigned to 276 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, with 22.3% having a homology with genes from F. fujikuroi. A total of 18,075 differentially expressed genes (DEGs) were identified, 720 of which were found to code for secreted proteins. Of these, 184 were identified as candidate effectors, while 79.89% had an upregulated expression. Moreover, 17 genes that were differentially expressed in RNA-seq studies were randomly selected for validation by quantitative real-time polymerase chain reaction (qRT–PCR). The study demonstrates that transcriptome analysis could be an effective method for identifying the repertoire of candidate effectors and may provide an invaluable resource for future functional analyses of F. proliferatum pathogenicity in F. proliferatum and tomato plant–host interactions.
Highlights
The tomato (Solanum lycopersicum) is one of the most important crop plants and one that servesas a model system for fruit development [1]
In order to obtain an overview of the F. proliferatum transcriptome and effector gene activity durinIng othrdeedritfoferoebnttaipnhaanseosvoefrviniefwectoiof nth, ewFe. uprsoeldifearatsucamnntrianngscerliepcttoromnemanicdroesfcfoepcteortogeonbesearcvteivsitiyx dsaumripngletshoefdFi.fpferroelinfetrpahtuamse-sinoffeicntefedcttioomn,awtoeleuasvedesaastcdainfnfeirnegnetliencftercotniomnitcirmosecpoopienttos (o0b,s1e2r,v2e4s,i4x8s,a7m2 panleds o96f Fh. parfotelirfeFra. tpurmol-iifnerfaetcutemditnoomcuatlaotlieoanv)e(sFiagtudrieff1er).enIrtriengfueclatiroenptiidmeermpoalinctesll(s0,w1e2r,e24fo, u48n,d72onanthde9s6e hleaafvteesr Fa.npdrotlhifeereaxttuemntinoof cthuilsatiirorneg) u(Flaigriutyreg1r)e.wIrrweigtuhlaarloenpgideerrimnfaelcctieolnls twimeree
This study is the first to report the use of transcriptome analysis as a means of screening effector genes that are involved in F. proliferatum-infected tomato interactions
Summary
The tomato (Solanum lycopersicum) is one of the most important crop plants and one that servesas a model system for fruit development [1]. Chinese tomato production is threatened by diseases Among these is tomato leaf spot caused by Fusarium proliferatum (Matsushima) Nirenberg, one of the most destructive fungal diseases for tomatoes [3]. Fusarium proliferatum is an endophyte that dwells on the plant and produces a large number of conidia that can survive for many years in the soil [11]. The F. oxysporum infection process in tomatoes has been well documented [12,13,14], but F. proliferatum’s infection process on tomatoes has not been as thoroughly described. This is an important problem that needs urgent solving
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
