Abstract
Fungicides are frequently detected in the water bodies, however, the adverse effects of these fungicides on aquatic lives remain limited. To better understand the adverse effects of amisulbrom (AML) and isoflucypram (ISO) on embryogenesis, zebrafish embryos were exposed to two different fungicides, 0.75 μM amisulbrom (AML) and 2.5 μM isoflucypram (ISO), for 72 h. Transcriptome sequencing was employed to identify differentially expressed genes (DEGs) after AML and ISO exposure. A total of 571 and 3471 DEGs were detected between the libraries of the two fungicides-treated groups and the control, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that PPAR signaling pathway, phototransduction, ribosome and p53 signaling pathway were significantly enriched in response to both AML and ISO stress. Moreover, a number of DEGs involved in tyrosine metabolism, phagosome pathway, cell cycle pathway, extracellular matrix (ECM) receptor interaction pathway, and arginine and proline metabolism were specially enriched after exposure to AML; a number of DEGs involved in notch signaling pathway, drug metabolism, alanine, aspartate and glutamate metabolism, amino-acyl−tRNA biosynthesis, and protein processing in endoplasmic reticulum were significantly enriched after exposure to ISO. These results provide novel insights into the toxicological mechanisms underlying fish’s responses to fungicides.
Highlights
Water Pollution of Life and Environmental Science, College of Life and Environmental Science, Engineering Research Center of Molecular Medicine of Ministry of Education, Key Laboratory of Fujian
The current analysis provides transcriptomic findings in relation to the toxicity associated with AML and ISO in zebrafish
The enrichment analyses imply that diverse cellular, metabolic or signaling pathways are affected in the zebrafish larvae after exposure to AML
Summary
Water Pollution of Life and Environmental Science, College of Life and Environmental Science, Engineering Research Center of Molecular Medicine of Ministry of Education, Key Laboratory of Fujian. Medicine and Molecular Diagnosis of Fujian Universities, School of Biomedical Sciences, Wenzhou Key Laboratory of Sanitary Microbiology, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Abstract: Fungicides are frequently detected in the water bodies, the adverse effects of these fungicides on aquatic lives remain limited. The mode of action of AML and ISO are based on the blockage of cytochrome-bc complex and succinate dehydrogenase involved in the mitochondrial electron transport system, respectively, leading to an inhibition of cellular respiration [1,3,4]. Both AML and ISO are potential persistent in the environment. The widespread use of AML and ISO fungicide may cause potential negative impacts on the environment
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