Abstract
Cf-12 is an effective gene for resisting tomato leaf mold disease caused by Cladosporium fulvum (C. fulvum). Unlike many other Cf genes such as Cf-2, Cf-4, Cf-5, and Cf-9, no physiological races of C. fulvum that are virulent to Cf-12 carrying plant lines have been identified. In order to better understand the molecular mechanism of Cf-12 gene resistance response, RNA-Seq was used to analyze the transcriptome changes at three different stages of C. fulvum infection (0, 4, and 8 days post infection [dpi]). A total of 9100 differentially expressed genes (DEGs) between 4 and 0 dpi, 8643 DEGs between 8 and 0 dpi and 2547 DEGs between 8 and 4 dpi were identified. In addition, we found that 736 DEGs shared among the above three groups, suggesting the presence of a common core of DEGs in response to C. fulvum infection. These DEGs were significantly enriched in defense-signaling pathways such as the calcium dependent protein kinases pathway and the jasmonic acid signaling pathway. Additionally, we found that many transcription factor genes were among the DEGs, indicating that transcription factors play an important role in C. fulvum defense response. Our study provides new insight on the molecular mechanism of Cf resistance to C. fulvum, especially the unique features of Cf-12 in responding to C. fulvum infection.
Highlights
IntroductionPassalora fulva) is a biotrophic pathogen of tomato (Solanum lycopersicum), which causes leaf mold disease (Cooke, 1883)
We investigated the transcriptome profiles of Cf12 tomato in response to C. fulvum infection using RNA-Seq
gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that many differentially expressed genes (DEGs) and their associated pathways are involved in disease resistance against fungal pathogens such as calcium dependent protein kinases (CDPK), wound salicylic acid inducible protein kinase (SIPK), respiratory burst oxidase homolog protein D/B (Rboh), heat shock protein 90 (Hsp90) and suppressor of G2 allele of SKP1 (SGT1; Piedras et al, 1998; Rivas and Thomas, 2005; Nekrasov et al, 2006; Hong et al, 2007)
Summary
Passalora fulva) is a biotrophic pathogen of tomato (Solanum lycopersicum), which causes leaf mold disease (Cooke, 1883). This fungus infects primarily the foliage, and occasionally the petioles and stems (Butler and Jones, 1949; Jones et al, 1997). C. fulvum has many physiological races, and new physiological races continue to be evolved (Westerink et al, 2004). These physiological races differ in race-specific elicitor proteins encoded by effector genes, and one of which is recognized as the AVR gene. An incompatible interaction (chlorosis) occurs when the plant is able to resist the pathogen and prevent infection, while a compatible interaction
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
