Abstract
Fusarium wilt is a destructive soil-borne disease that threatens the production of mung bean. Mung bean lines Zheng8-4 and Zheng8-20 show high resistance and high susceptibility to Fusarium wilt, respectively. Transcriptome analysis was carried out to identify candidate genes involved in Fusarium wilt resistance using Zheng8-4 and Zheng8-20 at 0, 0.5, 1, 2, and 4 days post inoculation (dpi). Differential expression analysis showed that 3,254 genes responded to pathogen infection and were differentially expressed in the resistant and susceptible lines. Weighted gene co-expression network analysis (WGCNA) was also performed to identify five modules highly correlated with Fusarium wilt resistance, in which 453 differentially expressed genes (DEGs) were considered likely to be involved in Fusarium wilt resistance. Among these DEGs, we found 24 genes encoding resistance (R) proteins, 22 encoding protein kinases, 20 belonging to transcription factor families, 34 encoding proteins with oxidoreductase activity, 17 involved in stimulation/stress responses, and 54 annotated to pathogen resistance-related pathways. Finally, 27 annotated genes were further selected as candidate genes of Fusarium wilt resistance in mung bean. This study identifies novel potential resistance-related genes against Fusarium wilt and provides a theoretical basis for further investigation of Fusarium wilt resistance in mung bean breeding.
Highlights
Mung bean (Vigna radiata L.) is an economically important grain legume crop used for human consumption
Mung bean lines Zheng8-4 and Zheng8-20 were separated from the Zhenglv8 cultivar, which is widely bred in China
These results confirmed that the Zheng8-4 line was highly resistant to Fusarium wilt and that the Zheng8-20 line was highly susceptible to Fusarium wilt
Summary
Mung bean (Vigna radiata L.) is an economically important grain legume crop used for human consumption. It is cultivated mostly in South, East and Southeast Asia for its edible seeds and sprouts (Schafleitner et al, 2015). The productivity and quality of mung bean are largely limited by various biotic stresses, and disease is among the main factors. Among these diseases, Fusarium wilt has become increasingly severe in most mung bean cultivation areas (Sun et al, 2019). Fusarium wilt is a destructive soil-borne disease caused by Fusarium oxysporum. F. oxysporum strains have a broad host range and have been divided into various formae speciales according to their host specificity
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