Transcriptome analysis of red sea bream (Pagrus major) head kidney and spleen infected by Vibrio anguillarum

Abstract

Red sea bream (Pagrus major) is described as one of the marine fishes with high economic value, which is widely cultivated in East Asia. With large-scale and intensive aquaculture, bacterial diseases outbreak more frequently than before. In order to effectively prevent and control Vibrio anguillarum disease in red sea bream, we systematically analyzed the immune response mechanism of the head kidney and spleen of P. major infected with Vibrio anguillarum by RNAseq technology. In this study, we obtained 225,106,036 clean reads. By using Nr, Nt, Pfam, KOG/COG, Swiss-prot, KEGG, GO databases, we annotated 85,595 genes. In the head kidney and spleen, we obtained 3963 and 4981 DEGs (different expressed genes) respectively. To further study the function of DEGs, we performed GO and KEGG enrichment analysis on them. In pathway enrichment, the pathways which significantly enriched in both head kidney and spleen included Antigen processing and presentation, Phagocytosis and Cell adhesion molecules. In the head kidney, Ubiquitin-mediated proteolysis was specially enriched; while Intestinal immune network for IgM production, Complement and coagulation cascade were specially enriched in the spleen. This study provide effective data support for the comprehensive analysis of mechanism in P. major immune response following V. anguillarum infection, which would lay the foundation for further disease prevention and treatment.