Transcriptome Analysis of Rainbow Trout (Oncorhynchus mykiss) Eggs Subjected to the High Hydrostatic Pressure Treatment

Artur Gurgul,Monika Bugno-Poniewierska,Klaudia Pawlina-Tyszko,Tomasz Szmatoła,Stefan Dobosz,Igor Jasielczuk,Konrad Ocalewicz

doi:10.1155/2018/5197126

Abstract

High hydrostatic pressure (HHP) causes depolymerization of the spindle microtubules. HHP applied to fish eggs prevents extrusion of the second polar body and inhibits the first cell cleavage, and it is used to produce triploids and diploid gynogenetic and androgenetic individuals. HHP has been also found to affect biomolecules including nucleic acids, and it may be presumed that HHP administered to the rainbow trout (Oncorhynchus mykiss) eggs disturbs cytoplasmic maternal RNA indispensable for the early embryogenesis. To verify this assumption, quality and quantity of RNA extracted from the rainbow trout eggs subjected to the high hydrostatic pressure shock were analyzed. Provided results exhibited that maternal transcriptome was resistant to a three-minute exposure to 65.5 MPa of HHP treatment. Some trend showing increase of the RNA integrity was observed in the HHP-treated eggs; however, the difference was not statistically significant. Alterations in the expression profiles in the rainbow trout eggs subjected to HHP were also negligible. Greater differences in the maternal gene expression were observed between eggs from different clutches than between HHP-treated and untreated eggs from the same clutch. It may be assumed that exposure to HHP shock was too short to modify significantly maternal transcripts in the rainbow trout eggs.

Highlights

High hydrostatic pressure (HHP) treatment may be lethal for the prokaryotic and eukaryotic cells
It has been observed that mammalian cells subjected to HHP of 40–60 MPa are more resistant to the cryopreservation process and thawed oocytes, spermatozoa, and embryos show increased stress tolerance and the postthaw survival [4, 5]
No clear evidence for the RNA degradation was detected in the rainbow trout eggs exposed to 65.5 MPa of HHP shock that lasted 3 minutes

Summary

Introduction

High hydrostatic pressure (HHP) treatment may be lethal for the prokaryotic and eukaryotic cells. HHP about 200 MPa causes apoptotic death of the mammalian cells while treatment with HHP higher than 300 MPa results in the cell death by necrosis [1]. HHP inactivates many pathogens and keeps unchanged functional and nutritional characteristics of many products. It has been widely used in the food industry to inactivate microorganisms [2, 3]. It has been observed that mammalian cells subjected to HHP of 40–60 MPa are more resistant to the cryopreservation process and thawed oocytes, spermatozoa, and embryos show increased stress tolerance and the postthaw survival [4, 5]

Methods

Results

Discussion

Conclusion