Abstract
BackgroundThe Pacific white shrimp, Litopenaeus vannamei, is a worldwide cultured crustacean species with important commercial value. Over the last two decades, Taura syndrome virus (TSV) has seriously threatened the shrimp aquaculture industry in the Western Hemisphere. To better understand the interaction between shrimp immune and TSV, we performed a transcriptome analysis in the hepatopancreas of L. vannamei challenged with TSV, using the 454 pyrosequencing (Roche) technology.Methodology/Principal FindingsWe obtained 126919 and 102181 high-quality reads from TSV-infected and non-infected (control) L. vannamei cDNA libraries, respectively. The overall de novo assembly of cDNA sequence data generated 15004 unigenes, with an average length of 507 bp. Based on BLASTX search (E-value <10−5) against NR, Swissprot, GO, COG and KEGG databases, 10425 unigenes (69.50% of all unigenes) were annotated with gene descriptions, gene ontology terms, or metabolic pathways. In addition, we identified 770 microsatellites and designed 497 sets of primers. Comparative genomic analysis revealed that 1311 genes differentially expressed in the infected shrimp compared to the controls, including 559 up- and 752 down- regulated genes. Among the differentially expressed genes, several are involved in various animal immune functions, such as antiviral, antimicrobial, proteases, protease inhibitors, signal transduction, transcriptional control, cell death and cell adhesion.Conclusions/SignificanceThis study provides valuable information on shrimp gene activities against TSV infection. Results can contribute to the in-depth study of candidate genes in shrimp immunity, and improves our current understanding of this host-virus interaction. In addition, the large amount of transcripts reported in this study provide a rich source for identification of novel genes in shrimp.
Highlights
Taura syndrome virus (TSV) is a contagious viral disease of penaeid shrimp [1]
Files containing these data were deposited in the Short Read Archive of the National Center for Biotechnology Information (NCBI) with accession numbers of SRR554365 (TSV-infected) and SRR556131
Among the differentially expressed genes homologous to antiviral proteins, we found that a homolog of Zinc finger CCCH-type antiviral protein was significantly up regulated in TSV-infected shrimp compared to non-infected controls
Summary
Taura syndrome virus (TSV) is a contagious viral disease of penaeid shrimp [1]. Over the last two decades, TSV has seriously threatened the shrimp aquaculture industry and caused serious economic losses [2,3]. In cultured Pacific white shrimp (Litopenaeus vannamei), which has become the major aquacultured crustacean species in the Western Hemisphere [4], TSV can cause a cumulative mortality ranged from 40 to .90% [5]. The survival shrimp of TSV infections may carry the virus for life [6,7]. The Pacific white shrimp, Litopenaeus vannamei, is a worldwide cultured crustacean species with important commercial value. Over the last two decades, Taura syndrome virus (TSV) has seriously threatened the shrimp aquaculture industry in the Western Hemisphere. To better understand the interaction between shrimp immune and TSV, we performed a transcriptome analysis in the hepatopancreas of L. vannamei challenged with TSV, using the 454 pyrosequencing (Roche) technology
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
