Transcriptome analysis of maize explant for anther culture: comparison of three different RNA extraction methods

Abstract

Breeding is one method to develop better varieties. This method can be accelerated by using haploid technology. The availability of haploid inducer lines in maize makes in-vivo techniques preferred, especially for temperate maize. Developing in-vitro through anther culture in haploid breeding is still needed since haploid inducer lines are still limited in tropical maize. Like many other crops facing recalcitrancy problems, maize also experiences the same, so cracking recalcitrant traits using transcriptomic approaches is necessary. In order to enhance the success of the sequencing, obtaining a high quantity and quality of RNA product is needed since it will affect the data. This study aimed to determine the best RNA extraction methods using three different kits of maize explant for anther culture with high purity and concentrations. RNA extraction in this study was performed using Tripure, RibospinTM, Promega, and Tripure kits. The results showed that all All the kits were able to produce high concetration of RNA, where the Ribospin (Geneall) performed the best (both for the concentration and purity). Meanwhile Tripure kit were only able to produced highest concentration of RNA, but lowest purity.