Abstract
Polyporus umbellatus, a species symbiotic with Armillaria mellea and it also exhibits substantial defence response to Armillaria mellea infection. There are no genomics resources databases for understanding the molecular mechanism underlying the infection stress of P. umbellatus. Therefore, we performed a large-scale transcriptome sequencing of this fungus with A. mellea infection using Illumina sequencing technology. The assembly of the clean reads resulted in 120,576 transcripts, including 38,444 unigenes. Additionally, we performed a gene expression profiling analysis upon infection treatment. The results indicated significant differences in the gene expression profiles between the control and the infection group. In total, 10933 genes were identified between the two groups. Based on the differentially expressed genes, a Gene Ontology annotation analysis showed many defence-relevant categories. Meanwhile, the Kyoto Encyclopedia of Genes and Genomes pathway analysis uncovered some important pathways. Furthermore, the expression patterns of 13 putative genes that are involved in defence response resulting from quantitative real-time PCR were consistent with their transcript abundance changes as identified by RNA-seq. The sequenced genes covered a considerable proportion of the P. umbellatus transcriptome, and the expression results may be useful to strengthen the knowledge on the defence response of this fungus defend against Armillaria mellea invasion.
Highlights
A cavity structure in the medullar tissue and isolate A. mellea to prevent further intrusion of A. mellea hyphae under SEM or TEM observation[10]
To obtain the P. umbellatus sclerotia transcriptome expression profile with A. mellea infection, one non-normalized library was constructed using sclerotia tissue with A. mellea infection and normal sclerotia tissue as control
Illumina sequencing data from P. umbellatus sclerotia was deposited in the NCBI SRA database under accession number SRP058382
Summary
A cavity structure in the medullar tissue and isolate A. mellea to prevent further intrusion of A. mellea hyphae under SEM or TEM observation[10]. Compared with the above screening techniques, RNA-Seq is known to have a wider dynamic range, higher technical reproducibility, and provide a better estimate of absolute expression levels[12] Along with these advantages, RNA-seq has been used to elucidate the response of plants to various environmental stresses, such as cold[13], salt[14,15] and drought[16,17]. In order to identify defence-related genes, potential defence-responsive genes were first identified based on Illumina tag-sequencing, and the differentially expressed genes (DEGs) were screened and further validated by qPCR. To our knowledge, this is the first large-scale assessment of Polyporus genomic information. Our results will facilitate understanding of the response of P. umbellatus to A. mellea infection
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