Abstract
BackgroundCucurbit chlorotic yellows virus (CCYV) is a recently reported bipartite crinivirus that causes chlorotic leaf spots and yellowing symptoms on the leaves of cucurbit plants. The virus–host interaction of CCYV remains to be elucidated, and the influence of criniviruses on the host gene transcriptome requires analysis.MethodsWe used transcriptome sequencing to analyse the differentially expressed genes (DEGs) caused by CCYV infection.ResultsCCYV infection resulted in 865 DEGs. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis identified 67 pathways, and the three major enrichment pathways (according to the P-values) were photosynthesis-antenna proteins (KO00196), phenylalanine metabolism (KO00360a), and phenylpropanoid biosynthesis (KO00940). Of the 13 DEGs identified in phenylalanine metabolism, 11 genes encode disease resistance-related phenylalanine ammonia-lyase (PAL) genes. Using quantitative real-time PCR, we validated the differential expression of 12 genes.ConclusionsOur study based on the CCYV–cucumber interaction provides comprehensive transcriptomic information, and will improve our understanding of host–crinivirus interactions.
Highlights
Cucurbit chlorotic yellows virus (CCYV) is a recently reported bipartite crinivirus that causes chlorotic leaf spots and yellowing symptoms on the leaves of cucurbit plants
The 3′ open reading frames (ORFs) of RNA1 are quite variable among the criniviruses; P6 and P22 show no significant similarity to corresponding proteins from other criniviruses [5]
Using quantitative real-time PCR, we validated the differential expression of 12 genes
Summary
Cucurbit chlorotic yellows virus (CCYV) is a recently reported bipartite crinivirus that causes chlorotic leaf spots and yellowing symptoms on the leaves of cucurbit plants. Cucurbit chlorotic yellows virus (CCYV) is a newly discovered cucurbit-infecting crinivirus of the family Closteroviridae [1,2,3,4,5,6,7]. CCYV causes chlorotic leaf spots and yellowing symptoms on the leaves of cucumber and melon, resulting in lower yields and poorer quality fruit. RNA1 contains four open reading frames (ORFs): ORF1a, ORF1b, ORF2, and ORF3. ORF1a encodes methyltransferase and RNA helicase, while ORF1b encodes an RNAdependent RNA polymerase motif. The 3′ ORFs of RNA1 are quite variable among the criniviruses; P6 and P22 show no significant similarity to corresponding proteins from other criniviruses [5]
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