Abstract
Female-sterile mutants are ideal materials for studying pistil development in plants. Here, we identified a female-sterile mutant fsm in Chinese cabbage. This mutant, which exhibited stable inheritance, was derived from Chinese cabbage DH line ‘FT’ using a combination of isolated microspore culture and ethyl methanesulfonate mutagenesis. Compared with the wild-type line ‘FT,’ the fsm plants exhibited pistil abortion, and floral organs were also relatively smaller. Genetic analysis indicated that the phenotype of fsm is controlled by a single recessive nuclear gene. Morphological observations revealed that the presence of abnormal ovules in fsm likely influenced normal fertilization process, ultimately leading to female sterility. Comparative transcriptome analysis on the flower buds of ‘FT’ and fsm using RNA-Seq revealed a total of 1,872 differentially expressed genes (DEGs). Of these, a number of genes involved in pistil development were identified, such as PRETTY FEW SEEDS 2 (PFS2), temperature-induced lipocalin (TIL), AGAMOUS-LIKE (AGL), and HECATE (HEC). Furthermore, GO and KEGG pathway enrichment analyses of the DEGs suggested that a variety of biological processes and metabolic pathways are significantly enriched during pistil development. In addition, the expression patterns of 16 DEGs, including four pistil development-related genes and 12 floral organ development-related genes, were analyzed using qRT-PCR. A total of 31,272 single nucleotide polymorphisms were specifically detected in fsm. These results contribute to shed light on the regulatory mechanisms underlying pistil development in Chinese cabbage.
Highlights
Floral organ development is the most obvious characteristic of the reproductive stage of flowering plants
Comparative transcriptome analysis of ‘FT’ and fsm showed that a number of DEGs are related to pistil development, and numerous DEGs involved in floral organ development were identified
The results provide a comprehensive view of the expression profiles of genes involved in pistil development, which may help uncover the molecular mechanisms determining the phenotypic differences between these lines
Summary
Floral organ development is the most obvious characteristic of the reproductive stage of flowering plants. The flowers of typical dicotyledonous plants are composed of four wheel-like structures (whorls). A variety of floral organ mutants have been characterized, leading to the isolation of a series of floral development- and morphogenesis-related genes using various techniques, and the expression patterns and functions of these genes have been analyzed. Studies on the commonality and characteristics of floral development in different species can help elucidate the origins and evolution of flowers. Such information would lay the foundation for altering the process of floral development, which might be used to control the flowering process and fertility in plants
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