Abstract
BackgroundOil flax (linseed, Linum usitatissimum L.) is one of the most important oil crops., However, the increases in drought resulting from climate change have dramatically reduces linseed yield and quality, but very little is known about how linseed coordinates the expression of drought resistance gene in response to different level of drought stress (DS) on the genome-wide level.ResultsTo explore the linseed transcriptional response of DS and repeated drought (RD) stress, we determined the drought tolerance of different linseed varieties. Then we performed full-length transcriptome sequencing of drought-resistant variety (Z141) and drought-sensitive variety (NY-17) under DS and RD stress at the seedling stage using single-molecule real-time sequencing and RNA-sequencing. Gene Ontology (GO) and reduce and visualize GO (REVIGO) enrichment analysis showed that upregulated genes of Z141 were enriched in more functional pathways related to plant drought tolerance than those of NY-17 were under DS. In addition, 4436 linseed transcription factors were identified, and 1190 were responsive to stress treatments. Moreover, protein-protein interaction (PPI) network analysis showed that the proline biosynthesis pathway interacts with stress response genes through RAD50 (DNA repair protein 50) interacting protein 1 (RIN-1). Finally, proline biosynthesis and DNA repair structural gene expression patterns were verified by RT- PCR.ConclusionsThe drought tolerance of Z141 may be related to its upregulation of drought tolerance genes under DS. Proline may play an important role in linseed drought tolerance by maintaining cell osmotic and protecting DNA from ROS damage. In summary, this study provides a new perspective to understand the drought adaptability of linseed.
Highlights
Oil flax is one of the most important oil crops., the increases in drought resulting from climate change have dramatically reduces linseed yield and quality, but very little is known about how linseed coordinates the expression of drought resistance gene in response to different level of drought stress (DS) on the genome-wide level
We hypothesized that the significant differences in gene expression patterns may be the reason for Z141 having better drought tolerance. From both Gene Ontology (GO) enrichment and MapMan analyses, we found that proline biosynthesis genes were significantly upregulated when Z141 and NY-17 were under DS
Our results revealed that a group of genes involved in plant drought tolerance were upregulated in only the linseed variety with better drought tolerance under DS
Summary
Oil flax (linseed, Linum usitatissimum L.) is one of the most important oil crops., the increases in drought resulting from climate change have dramatically reduces linseed yield and quality, but very little is known about how linseed coordinates the expression of drought resistance gene in response to different level of drought stress (DS) on the genome-wide level. Drought stress (DS) is the most prevalent environmental factor limiting crop productivity and can directly result in an average yield loss of more than 50%, and global climate change is increasing the frequency of severe drought conditions [1]. Plants have evolved a series of special mechanisms to resist the damage caused by DS. Tolerant maize varieties exhibited more drastic changes in global gene expression than susceptible varieties which correlated with different physiological mechanisms of adaptation to drought [5]. The mechanisms by which plants resist DS are still unclear
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