Abstract
The pH and salinity balance mechanisms of crayfish are controlled by a set of transport-related genes. We identified a set of the genes from the gill transcriptome from a freshwater crayfish Cherax quadricarinatus using the Illumina NGS-sequencing technology. We identified and characterized carbonic anhydrase (CA) genes and some other key genes involved in systematic acid-base balance and osmotic/ionic regulation. We also examined expression patterns of some of these genes across different sublethal pH levels [1]. A total of 72,382,710 paired-end Illumina reads were assembled into 36,128 contigs with an average length of 800 bp. About 37% of the contigs received significant BLAST hits and 22% were assigned gene ontology terms. These data will assist in further physiological-genomic studies in crayfish.
Highlights
Transcriptome analysis and characterisation of gill-expressed carbonic anhydrase and other key osmoregulatory genes in freshwater crayfish Cherax quadricarinatus
The pH and salinity balance mechanisms of crayfish are controlled by a set of transport-related genes
We identified a set of the genes from the gill transcriptome from a freshwater crayfish Cherax quadricarinatus using the Illumina NGS-sequencing technology
Summary
Through the sequencing of Cherax quadricarinatus gill transcriptome library, we obtained over 72 million (72,382,710) good quality paired-end sequence reads (90 bb each) after the removal of low quality reads. Total number of contigs: N50 N75 N25 Mean contig length Minimum contig length Length of the longest contig Number of contigs longer than 500 bp Number of contigs longer than 1500 bp GC content
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