Abstract
Transcriptional up-regulation of TERT and TERC necessarily not mean higher telomerase activity: A report of telomerase inhibition with higher transcription of TERT, TERC and TERF2 in A549 cells treated with staurosporine.
Highlights
Telomerase, a ribonucleoprotein complex that maintains telomere length, is highly activated in more than 90% tumor cells and generally absent in somatic cells
Telomerase consists of an RNA component (TR or TERC) that acts as template for synthesis of telomere DNA, and a reverse transcriptase unit (TERT) having catalytic activity of formation of telomeric DNA copying RNA template
Telomere shortening after each cycle of cell division can be prevented either by telomerase or by recombination-based mechanism known as alternative lengthening of telomere (ALT) [4, 5]
Summary
Telomerase, a ribonucleoprotein complex that maintains telomere length, is highly activated in more than 90% tumor cells and generally absent in somatic cells. Telomerase consists of an RNA component (TR or TERC) that acts as template for synthesis of telomere DNA, and a reverse transcriptase unit (TERT) having catalytic activity of formation of telomeric DNA copying RNA template. A number of small molecules inhibit catalytic activity and reduce transcription of TERT and thereby kill cancer cells [13, 14]. We present the data to resolve whether telomerase inhibition and apoptosis induction by staurosporine is independent to each other As such there is no report of its effect in telomere-associated proteins and telomere native structure. We report role of staurosporine in regulation of telomerase and telomere proteins in A549 cells. Other molecular biology grade fine chemicals were procured locally from SRL, India
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