Abstract
Background The term “persisters” refers to a small bacterial population that persists during treatment with high antibiotic concentration or dose in the absence of genetic resistance. The present study was designed to investigate the transcriptional response in indigenous Klebsiella pneumoniae under the ciprofloxacin stress. Methods Isolation and identification of K. pneumoniae were carried out through standard microbiological protocols. The characterization of quinolone resistance was performed by estimating the quinolone susceptibility testing, MIC estimation, and detecting the QRDR and PMQR. Transcriptional response of the isolates to ciprofloxacin was determined using qPCR. Results Among 34 isolates, 23 (67%) were resistant to ciprofloxacin. Both QRDR (gyrA and gyrB) and PMQR (qnrA, qnrB, and qnrS) were detected in the isolates, and all were found resistant to ciprofloxacin. The mRNA levels of both mutS and euTu under the influence of ciprofloxacin were significantly increased. On ciprofloxacin exposure, the mRNA levels of the DNA damage response element (mutS) were raised in a time-dependent fashion. K. pneumoniae showed high-level resistance to ciprofloxacin in the presence of mutations in QRDR and PMQR genes. Conclusion The transcriptional response revealed the upregulation of DNA repair and protein folding elements (mutS and euTu) in ciprofloxacin stress and delayed cell division. The ciprofloxacin was found to trigger various stress responses in a time- and concentration-dependent manner.
Highlights
Roman Farooq Alvi,1 Bilal Aslam,1 Muhammad Hidayat Rasool,1 Saima Muzammil,1 Abu Baker Siddique,1 Nafeesa Yasmeen,2 Mohsin Khurshid,1 Noreen Sarwar,3 Ahmad Almatroudi,4 Riaz Hussain,5 and Zulqarnain Baloch 6
Background. e term “persisters” refers to a small bacterial population that persists during treatment with high antibiotic concentration or dose in the absence of genetic resistance. e present study was designed to investigate the transcriptional response in indigenous Klebsiella pneumoniae under the ciprofloxacin stress
Both Quinolone resistance-determining regions (QRDRs), as well as plasmid-mediated quinolone resistance (PMQR), were detected in isolates that were found resistant to ciprofloxacin (Table 2)
Summary
Roman Farooq Alvi, Bilal Aslam ,1 Muhammad Hidayat Rasool ,1 Saima Muzammil ,1 Abu Baker Siddique ,1 Nafeesa Yasmeen, Mohsin Khurshid ,1 Noreen Sarwar, Ahmad Almatroudi ,4 Riaz Hussain, and Zulqarnain Baloch 6. E term “persisters” refers to a small bacterial population that persists during treatment with high antibiotic concentration or dose in the absence of genetic resistance. E present study was designed to investigate the transcriptional response in indigenous Klebsiella pneumoniae under the ciprofloxacin stress. 1. Introduction e term “persisters” refers to a small bacteria population that persists during treatment with high antibiotics concentration or dose in the absence of genetic resistance [1]. Sublethal antibiotic concentration is one of the potential factors associated with the development of persistent resistant bacterial strains [2]. Bacteria possess a strong defense mechanism against the antibiotics, which triggers the induction of various adaptive responses and reactions at various stages of the cell cycle. We have reported the molecular mechanism of resistance against ciprofloxacin in indigenous Klebsiella pneumoniae isolates [11]. e present study is designed to investigate the transcriptional response in indigenous Klebsiella pneumoniae under the stress of ciprofloxacin, which is helpful to find out the link between ciprofloxacin stress and its resistance in indigenous Klebsiella pneumoniae isolates
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Schedule a callMore From: The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale
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