Abstract
While somatic cell nuclear transfer (SCNT) techniques have been successfully implemented in several species to produce cloned embryos and offspring, the efficiencies of the procedures are extremely low, possibly due to insufficient reprogramming of somatic nuclei. Employing GeneChip microarrays, we describe global gene expression analysis of bovine in vitro fertilized (IVF) and SCNT blastocysts as well as respective donor cell lines to characterize differences in their transcription profiles. Gene expression profiles of our donor cell lines were significantly different from each other; however, the SCNT and IVF blastocysts displayed surprisingly similar gene expression profiles, suggesting that a major reprogramming activity had been exerted on the somatic nuclei. Despite this remarkable phenomenon, a small set of genes appears to be aberrantly expressed and may affect critical developmental processes responsible for the failures observed in SCNT embryos. Our data provide the most comprehensive transcriptome database of bovine IVF and SCNT blastocysts to date.
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