Abstract
BackgroundLonicera japonica Thunb. flower has been used for the treatment of various diseases for a long time and attracted many studies on its potential effects. Transcription factors (TFs) regulate extensive biological processes during plant development. As the restricted reports of L. japonica on TFs, our work was carried out to better understand the TFs’ regulatory roles under different developmental stages in L. japonica.ResultsIn this study, 1316 TFs belonging to 52 families were identified from the transcriptomic data, and corresponding expression profiles during the L. japonica flower development were comprehensively analyzed. 917 (69.68%) TFs were differentially expressed. TFs in bHLH, ERF, MYB, bZIP, and NAC families exhibited obviously altered expression during flower growth. Based on the analysis of differentially expressed TFs (DETFs), TFs in MYB, WRKY, NAC and LSD families that involved in phenylpropanoids biosynthesis, senescence processes and antioxidant activity were detected. The expression of MYB114 exhibited a positive correlation with the contents of luteoloside; Positive correlation was observed among the expression of MYC12, chalcone synthase (CHS) and flavonol synthase (FLS), while negative correlation was observed between the expression of MYB44 and the synthases; The expression of LSD1 was highly correlated with the expression of SOD and the total antioxidant capacity, while the expression of LOL1 and LOL2 exhibited a negative correlation with them; Many TFs in NAC and WRKY families may be potentially involved in the senescence process regulated by hormones and reactive oxygen species (ROS). The expression of NAC19, NAC29, and NAC53 exhibited a positive correlation with the contents of ABA and H2O2, while the expression of WRKY53, WRKY54, and WRKY70 exhibited a negative correlation with the contents of JA, SA and ABA.ConclusionsOur study provided a comprehensive characterization of the expression profiles of TFs during the developmental stages of L. japonica. In addition, we detected the key TFs that may play significant roles in controlling active components biosynthesis, antioxidant activity and flower senescence in L. japonica, thereby providing valuable insights into the molecular networks underlying L. japonica flower development.
Highlights
Lonicera japonica Thunb. flower has been used for the treatment of various diseases for a long time and attracted many studies on its potential effects
Contents of chlorogenic acid (CGA) and luteoloside in different developmental stages of L.japonica flower The contents of CGA and luteoloside in S1, S2, S3, S4 and S5 of L.japonica flower were measured by HPLC (Fig. 1)
The content of CGA exhibited an increase from S1 to S3, while reached its minimum at S4, and increased again at S5; The content of luteoloside increased from S1 to S3, followed by a decrease from S3 to S5
Summary
Lonicera japonica Thunb. flower has been used for the treatment of various diseases for a long time and attracted many studies on its potential effects. Recent studies indicated that polysaccharides from L. japonica flower buds show hypoglycemic and hypolipidemic effects on streptozotocininduced diabetic rats, and neuroprotective effects on cerebral ischemia-reperfusion injuries in rats [7, 8]. These findings demonstrated that, L. japonica has received much interest in recent years in specialized pharmacological research studies. Studies have been conducted to analyze the biosynthesis of active compounds by transcriptome [12,13,14], the regulatory mechanism of secondary metabolic pathways and the physiological processes during different developmental stages of L. japonica remain largely unknown
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