Abstract
BackgroundOur previous study has demonstrated that the transcription of AchnKCS involved in suberin biosynthesis was up-regulated by exogenous abscisic acid (ABA) during the wound suberization of kiwifruit, but the regulatory mechanism has not been fully elucidated.ResultsThrough subcellular localization analysis in this work, AchnbZIP29 and AchnMYB70 transcription factors were observed to be localized in the nucleus. Yeast one-hybrid and dual-luciferase assay proved the transcriptional activation of AchnMYB70 and transcriptional suppression of AchnbZIP29 on AchnKCS promoter. Furthermore, the transcription level of AchnMYB70 was enhanced by ABA during wound suberization of kiwifruit, but AchnbZIP29 transcription was reduced by ABA.ConclusionsTherefore, it was believed that ABA enhanced the transcriptional activation of AchnMYB70 on AchnKCS by increasing AchnMYB70 expression. On the contrary, ABA relieved the inhibitory effect of AchnbZIP29 on transcription of AchnKCS by inhibiting AchnbZIP29 expression. These results gave further insight into the molecular regulatory network of ABA in wound suberization of kiwifruit.
Highlights
Our previous study has demonstrated that the transcription of AchnKCS involved in suberin biosynthesis was up-regulated by exogenous abscisic acid (ABA) during the wound suberization of kiwifruit, but the regulatory mechanism has not been fully elucidated
Amino acid sequence homology Through the promoter sequence analysis by PlantCARE and bioinformatics searching by National Center of Biotechnology Information (NCBI) BLAST software, a bZIP (Achn340751) and an MYB (Achn117821) transcription factor were inferred to be downstream responses of ABA signaling and be associated with suberin biosynthesis based on the involvement of their close homologs in ABA responding and mechanical stress [33,34,35,36,37,38]
It showed that AchnbZIP29 and AchnMYB70 respectively belonged to Group I of bZIP transcription factors and R2R3-MYB 22 subgroup, which involved in the regulation of fatty acid biosynthesis [39,40,41]
Summary
Our previous study has demonstrated that the transcription of AchnKCS involved in suberin biosynthesis was up-regulated by exogenous abscisic acid (ABA) during the wound suberization of kiwifruit, but the regulatory mechanism has not been fully elucidated. Suberin is a plant cellwall biopolymer composed of glycerol-based aliphatic polyester and the associated polymeric aromatics [7, 8]. It is biosynthesized initially from the acylation of fatty acids by long chain acyl-CoA synthetase (LACS), following fatty acyl elongation controlled by fatty acid elongation enzyme complex (FAE), acyl reduction by fatty acyl reductase (FAR), fatty acyl oxidation by cytochrome P450 enzyme (CYP) and esterification of ω-hydroxy fatty acids and α, ω-dicarboxylic acids by glycerol 3-phosphate
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