Abstract
A comprehensive study of the transcriptional regulation of jasmonate metabolism, perception and signaling by heat, cold stress, and heat-induced chilling tolerance in ‘Fortune’ mandarin was performed. Changes in the expression of a set of 22 relevant jasmonate-related genes were evaluated during fruit storage at a chilling (2 °C) and a non-chilling control temperature (12 °C); and during fruit storage at 2 °C after being heat-conditioned (37 °C for 3 d). The changes occurring in response to heat (37 °C) were also examined. The results indicated that the genes participating in cis-(+)− 12-oxo-phytodienoic (OPDA) (CsLOX1, CsAOS, CsAOC3) and jasmonic acid (JA) (CsOPR2) synthesis would be, among the cold-responsive genes, the best markers of chilling stress leading to peel damage in citrus fruit. Most jasmonate-related genes are regulated by heat, but the genes regulated by cold stress in the heated-fruit, respect to the unheated, may be especially relevant for heat-induced chilling tolerance. Major effects of heat+cold, as compared to cold, were found in the expression of genes participating in the biosynthesis of OPDA (CsLOX5 and CsAOC3), JA (CsOPR3 and CsKAT2) and jasmonoyl-isoleucine (JA-Ile) (CsJAR1, CsCYP94B3 and CsCYP94C); but also in the expression of genes involved in JA perception (CsCOI1) and signaling, including the jasmonate transcriptional activator CsMYC2 and abundant negative regulators (CsJAZs and CsTOPLESS).
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