Abstract
The transcriptional regulator UlaR belongs to the family of PRD-containing transcriptional regulators, which are mostly involved in the regulation of carbohydrate metabolism. The role of the transcriptional regulator UlaR in Streptococcus pneumoniae has recently been described [1]. Here, we report detailed genome-wide transcriptional profiling of UlaR-regulated genes in S. pneumoniae D39 and its ∆ulaR derivative, either in the presence of 10 mM ascorbic acid in M17 medium using microarray analysis. 10 mM concentration of ascorbic acid was supplemented to the M17 medium because our lacZ-fusion studies indicated that UlaR acts as a transcriptional activator of its targets in the presence of ascorbic acid and the expression of the ula operon was maximal at a 10 mM ascorbic acid concentration [1]. All transcriptional profiling data of UlaR-regulated genes was deposited to Gene Expression Omnibus (GEO) database under accession number GSE61649.
Highlights
We report detailed genome-wide transcriptional profiling of UlaR-regulated genes in S. pneumoniae D39 and its ΔulaR derivative, either in the presence of 10 mM ascorbic acid in M17 medium using microarray analysis. 10 mM concentration of ascorbic acid was supplemented to the M17 medium because our lacZ-fusion studies indicated that UlaR acts as a transcriptional activator of its targets in the presence of ascorbic acid and the expression of the ula operon was maximal at a 10 mM ascorbic acid concentration [1]
We investigated the impact of an ulaR mutation on the transcriptional profile of S. pneumoniae D39
Transcriptome comparison of the D39 ΔulaR with the D39 wild-type grown in AM17 (10 mM Ascorbic acid + M17) revealed the specific effect on the gene expression of S. pneumoniae. 12 genes were downregulated and 9 genes were upregulated in the D39 ΔulaR strain compared to the D39 wild-type in the presence of ascorbic acid (Table 2)
Summary
Transcriptional profiling of UlaR-regulated genes in Streptococcus pneumoniae Shafeeq, Sulman; Afzal, Muhammad; Henriques-Normark, Birgitta; Kuipers, Oscar P. Document Version Publisher's PDF, known as Version of record. Citation for published version (APA): Shafeeq, S., Afzal, M., Henriques-Normark, B., & Kuipers, O. More information can be found on the University of Groningen website: https://www.rug.nl/library/open-access/self-archiving-pure/taverneamendment. Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. For technical reasons the number of authors shown on this cover page is limited to 10 maximum
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