Abstract
BackgroudEncephalomyocarditis virus (EMCV) has been discovered on pig farms worldwide and can cause myocarditis in piglets and reproductive failure in sows. However, little is known about the host transcriptional responses to infection and host-pathogen interactions.MethodsIn this study, transcription profiling was performed by Illumina RNA-Sequencing (RNA-seq) to identify EMCV induced differentially expressed genes in BHK-21 cells at serial time points (12, 24, and 30 h post infection (hpi)), using mock infected cells as control.ResultsWe identified 237, 241, and 207 differentially expressed genes (DEGs) respectively, majority of which were up-regulated. A large number of DEGs clustered into host defense, cellular signaling and metabolism categories. Moreover, short time series expression analysis revealed that 12 hpi was an important time point for expression change, indicating host virus resistance.ConclusionsThis RNA-seq analysis provides the first data for understanding the network of virus host interactions under EMCV infection in vitro, and for identifying host components which involved in the virus infection course.
Highlights
We identified 237, 241, and 207 differentially expressed genes (DEGs) respectively, majority of which were up-regulated
The number of Encephalomyocarditis virus (EMCV) strains isolated from pigs and wild animals in China is increasing in recent years [7,8,9]
We investigated the host responses induced by EMCV at different time points (12, 24 and 30 hpi) using RNA-seq, and analyzed differential gene expression between uninfected and infected cell groups, as well as the dynamic of host gene expression during the virus infection
Summary
Transcription profiling was performed by Illumina RNA-Sequencing (RNA-seq) to identify EMCV induced differentially expressed genes in BHK-21 cells at serial time points (12, 24, and 30 h post infection (hpi)), using mock infected cells as control
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