Abstract
The stuctural organization of selected regions on mustard plastid DNA that hybridize with in vivio RNA has been investigated. Map positions as well as the orientations of several genes within restriction fragments of the large single copy region have been determined. Cross-hybridization with spinach gene probes shows these to include the gene for the P700 apoprotein of PSI, the genes for cytochromes b6 and f and the genes for the alpha (and possibly CF0-III) subunit of the ATP synthase complex. All except one of these genes are colinear with their mature in vivo RNA. The putative cytochrome f gene seems to be split. Northern analysis of several genes has revealed multiple RNA species, indicating a complex transcriptional organization. Transcribed regions, of unknown function, have also been located and mapped within the small single copy region of mustard plastid DNA.
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