Transcriptional mapping of the promoter of the aminoglycoside acetyltransferase gene ( aacC9) of neomycin-producing Micromonospora chalcea

Abstract

We have studied the promoter of the gene encoding aminoglycoside acetyltransferase ( aacC9) in neomycin-producing Micromonospora chalcea. S1 nuclease mapping showed that the transcription initiation point of this gene is at the translation start point, with no evidence of a conventional ribosome-binding site. The aac of paromycin-producing Streptomyces rimosus forma paromomycinus shows the same characteristics; there is no homology in the promoter regions of the two genes, whereas the coding sequences are very similar.