Abstract
The freshwater crayfish Procambarus clarkii is an animal model employed for physiological and immunological studies and is also of great economic importance in aquaculture. Although it is a species of easy husbandry, a high percentage of its production is lost annually as a result of infectious diseases. Currently, genetic information about the immune system of crustaceans is limited. Therefore, we used the abdominal nerve cord from P. clarkii to obtain its transcriptome using Next Generation Sequencing (NGS) to identify proteins that participate in the immune system. The reads were assembled de novo and consensus sequences with more than 3000 nucleotides were selected for analysis. The transcripts of the sequences of RNA were edited for annotation and sent to the GenBank database of the National Center for Biotechnology Information (NCBI). We made a list of accession numbers of the sequences which were organized by the putative role of the immune system pathway in which they participate. In this work, we report on 80 proteins identified from the transcriptome of crayfish related to the immune system, 74 of them being the first reported for P. clarkii. We hope that the knowledge of these sequences will contribute significantly to the development of future studies of the immune system in crustaceans.
Highlights
Decapod crustaceans show a high capacity for living in diverse environments
We identified several proteins that participate in the immune system; we performed a manual Basic Local Alignment Search Tool (BLAST) search for the obtained transcripts and identified several molecules involved in the immunity system of invertebrates
We identified 80 mRNA molecules that were related to the immune system of decapods and arthropods
Summary
The freshwater crayfish Procambarus clarkii is a species widely used in physiological studies in diverse laboratories and as a biomarker in contaminated ecosystems [1]; it can even be a predator [2,3], which suggests that it is an adaptable organism with an efficient immune system. Procambarus clarkii has been considered one of the three viable species as a model of crustaceans [4]. It is one of the most farmed shrimp species for human consumption, with production estimated in 2014 by the Food and Agriculture Organization at 6.9 million tons of crustaceans (USD 36.2 billion), of which 723,288 tons corresponded to the cultivation of P. clarkii [5]. Because of the economic value of its production worldwide, it is important to limit and effectively control infections that can diminish its profitability
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