Abstract
The Japanese scallop (Mizuhopecten yessoensis) is one of the most important aquaculture species in Asian countries; however, it has suffered severe infection by Polydora in northern China in recent years, causing great economic losses. The Polydora parasitizes the shell of scallops, badly destroying the shell’s structure. To investigate the molecular response mechanism of M. yessoensis to Polydora infestion, a comprehensive and niche-targeted cDNA sequence database for diseased scallops was constructed. Additionally, the transcriptional changes in the edge mantle, central mantle and hemocytes, tissues directly related to the disease, were first described in this study. The results showed that genes involved in shell formation and immunomodulation were significantly differentially expressed due to the infestation. Different transcriptional changes existed between the two mantle regions, indicating the different molecular functions likely responsible for the formation of different shell layers. The differential expression of genes for immune recognition, signal transduction and pathogen elimination presented an integrated immune response process in scallops. Moreover, neuromodulation and glycometabolism involved in the regulation process with relevant function significantly enriched. The study provides valuable information for mechanism study of shell formation and immunomodulation in scallops.
Highlights
Polydora[6,7,8,9,10,11]; the response of M. yessoensis, especially on the molecular level, has never been reported, limiting the understanding of disease resistance in scallops
Transcriptional changes in the mantle tissue of Ruditapes philippinarum caused by Brown Ring Disease include genes related to biomineralization and many immunity genes[24,25], implying the important role of mantle tissue in the immune response to pathogens
A comprehensive and niche-targeted cDNA sequences database was constructed for M. yessoensis infested by Polydora, transcriptional changes in the edge mantle, central mantle and hemocytes of the diseased scallops were first detected, and genes related to shell formation and immunity were screened
Summary
Polydora[6,7,8,9,10,11]; the response of M. yessoensis, especially on the molecular level, has never been reported, limiting the understanding of disease resistance in scallops. As the classical immune tissue in scallops, hemocytes use encapsulation and phagocytosis to eliminate undesirable particles (e.g., pathogens and abnormal/dead cells) performing the cellular immune reactions and contribute to the secretion of immune macromolecules to execute humoural immune responses, such as immune recognition, signal transduction and destruction of invaders, as reviewed by Song et al.[27]. A comprehensive and niche-targeted cDNA sequences database was constructed for M. yessoensis infested by Polydora, transcriptional changes in the edge mantle, central mantle and hemocytes of the diseased scallops were first detected, and genes related to shell formation and immunity were screened. The aim was to reveal the molecular response mechanism of M. yessoensis to the infestation of Polydora, and provide more valuable information for the mechanism studies of biomineralization and immunomodulation
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