Abstract
BackgroundRice is highly sensitive to chilling stress during the seedling stage. However, the adaptable photo-thermo sensitive genic male sterile (PTGMS) rice line, Yu17S, exhibits tolerance to low temperatures. Currently, the molecular characteristics of Yu17S are unclear.ResultsTo evaluate the molecular mechanisms behind cold responses in rice seedlings, a comparative transcriptome analysis was performed in Yu17S during seedling development under normal temperature and low temperature conditions. In total, 9317 differentially expressed genes were detected. Gene ontology and pathway analyses revealed that these genes were involved mostly in photosynthesis, carotenoid biosynthesis, carbohydrate metabolism and plant hormone signal transduction. An integrated analysis of specific pathways combined with physiological data indicated that rice seedlings improved the performance of photosystem II when exposed to cold conditions. Genes involved in starch degradation and sucrose metabolism were activated in rice plants exposed to cold stress treatments, which was accompanied by the accumulation of soluble sugar, trehalose, raffinose and galactinol. Furthermore, chilling stress induced the expression of phytoene desaturase, 15-cis-ζ-carotene isomerase, ζ-carotene desaturase, carotenoid isomerase and β-carotene hydroxylase; this was coupled with the activation of carotenoid synthase activity and increases in abscisic acid (ABA) levels in rice seedlings.ConclusionsOur results suggest that Yu17S exhibited better tolerance to cold stress with the activation of carotenoid synthase activity and increasing of ABA levels, and as well as the expression of photosynthesis-related genes under cold condition in rice seedlings.
Highlights
Rice (Oryza sativa L.) is one of the most important food crops in the world and feeds more than 50% of the world’s population [1, 2]
To better understand the mechanisms driving Yu17S cold tolerance during the seedling stage, we investigated the transcriptomic changes in Yu17S seedlings subjected to cold stress using RNA sequencing (RNA-Seq)
Chemical and transcriptomic data, we found that genes related to carotenoid and abscisic acid (ABA) biosynthesis, starch degradation, sucrose metabolism and photosynthesis were differentially expressed in coldstressed Yu17S
Summary
Rice (Oryza sativa L.) is one of the most important food crops in the world and feeds more than 50% of the world’s population [1, 2]. Rice is more sensitive to cold stress than other cereal crops such as wheat Rice seedling establishment is negatively affected by. Pan et al BMC Plant Biology (2022) 22:44 areas of China prefer to grow Indica rice cultivars [8]. High-yield Indica rice cultivars possessing cold stress tolerance are highly desirable in these regions. Rice is highly sensitive to chilling stress during the seedling stage. The adaptable photothermo sensitive genic male sterile (PTGMS) rice line, Yu17S, exhibits tolerance to low temperatures. The molecular characteristics of Yu17S are unclear
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