Transcriptional Analysis of Microcystis aeruginosa Co-Cultured with Algicidal Bacteria Brevibacillus laterosporus.

Yulei Zhang,Xianghu Huang,Changling Li,Feng Li,Ning Zhang,Dong Chen,Qianru Li,Xiaoxia Luo

doi:10.3390/ijerph18168615

Yulei Zhang, Xianghu Huang + Show 6 more

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https://doi.org/10.3390/ijerph18168615

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Abstract

Harmful algal blooms caused huge ecological damage and economic losses around the world. Controlling algal blooms by algicidal bacteria is expected to be an effective biological control method. The current study investigated the molecular mechanism of harmful cyanobacteria disrupted by algicidal bacteria. Microcystis aeruginosa was co-cultured with Brevibacillus laterosporus Bl-zj, and RNA-seq based transcriptomic analysis was performed compared to M. aeruginosa, which was cultivated separately. A total of 1706 differentially expressed genes were identified, which were mainly involved in carbohydrate metabolism, energy metabolism and amino acid metabolism. In the co-cultured group, the expression of genes mainly enriched in photosynthesis and oxidative phosphorylation were significantly inhibited. However, the expression of the genes related to fatty acid synthesis increased. In addition, the expression of the antioxidant enzymes, such as 2-Cys peroxiredoxin, was increased. These results suggested that B. laterosporus could block the electron transport by attacking the PSI system and complex I of M. aeruginosa, affecting the energy acquisition and causing oxidative damage. This further led to the lipid peroxidation of the microalgal cell membrane, resulting in algal death. The transcriptional analysis of algicidal bacteria in the interaction process can be combined to explain the algicidal mechanism in the future.

Highlights

With the increase in water eutrophication, harmful algal blooms (HABs) have frequently occurred worldwide
The Algicidal Efficiency control group (BG11), the chlorophyll a concentrat ginosa significantly decreased with the addition of B. laterosporus from th Compared with the control group (BG11), the chlorophyll a concentration of M
We provide the molecular responses of M. aeruginosa to B. laterosporus treatment

Summary

Introduction

With the increase in water eutrophication, harmful algal blooms (HABs) have frequently occurred worldwide. In HABs, cyanobacteria are the most common group of microalgae, which can produce toxins [1,2,3]. Cyanobacterial blooms can cause serious water environmental problems, such as water oxygen deprivation, odorous substances and toxins, which are harmful to drinking water, aquatic animals, even threatening to human health [4,5,6]. Various methods have been proposed for removing or inhibiting cyanobacterial blooms, which include physical, chemical and biological methods. Physical and chemical methods are expensive and cause secondary pollution, which is potentially harmful to aquatic ecosystems [7]. Biological methods are effective strategies to control HABs [8]

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