Abstract
The comparative transcriptional analysis of highly syntenic regions in six different organ types between Medicago truncatula (barrel medic) and Glycine max (soybean), using nucleotide tiling microarrays, provides insights into genome organization and transcriptional regulation in these legume plants.
Highlights
Legumes are the third largest family of flowering plants and are unique among crop species in their ability to fix atmospheric nitrogen
Our results provide transcriptional support to over 80% of the predicted genes and identified 499 and 660 transcriptionally active region (TAR) from barrel medic and soybean, respectively
Genome tiling microarray analysis is emerging as a new powerful approach, which involves the development of tiling arrays containing progressive oligonucleotide tiles that represent a target genome
Summary
Legumes are the third largest family of flowering plants and are unique among crop species in their ability to fix atmospheric nitrogen. As a result of recent genome sequencing efforts, legumes are one of a few plant families with extensive genomic and transcriptomic data available in multiple species. Genome tiling arrays have been used in a number of model species for which the full genome sequence is available [3,4,5,6,7,8] Results from these studies have shown that for well-documented transcripts, such as those of polyadenylated RNAs from annotated genes, hybridization signals from tiling arrays identify the transcriptional start and stop sites, the locations of introns, and the events of alternative splicing [3,4,5,6,7,8]. Tiling array signals reveal a large number of putative novel transcripts for which no conventional explanations are yet available
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