Transcriptional analysis of Bemisia tabaci MEAM1 cryptic species under the selection pressure of neonicotinoids imidacloprid, acetamiprid and thiamethoxam

Cheng Song Zhou,Rui Xingyue Zhang,Qian Cao,Xiao Hu Guo,De Ying Ma,Huan Huan Lv

doi:10.1186/s12864-021-08241-6

Cheng Song Zhou, Rui Xingyue Zhang + Show 4 more

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https://doi.org/10.1186/s12864-021-08241-6

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Journal: BMC Genomics	Publication Date: Jan 5, 2022
Citations: 5	License type: open-access

Affiliation: Xinjiang Agricultural University

Abstract

BackgroundNeonicotinoids are widely applied in the control of the destructive agricultural pest Bemisia tabaci, and resistance against these chemicals has become a common, severe problem in the control of whiteflies. To investigate the molecular mechanism underlying resistance against nenonicotinoids in whiteflies, RNA-seq technology was applied, and the variation in the transcriptomic profiles of susceptible whiteflies and whiteflies selected by imidacloprid, acetamiprid and thiamethoxam treatment was characterized.ResultsA total of 90.86 GB of clean sequence data were obtained from the 4 transcriptomes. Among the 16,069 assembled genes, 584, 110 and 147 genes were upregulated in the imidacloprid-selected strain (IMI), acetamiprid-selected strain (ACE), and thiamethoxam (THI)-selected strain, respectively, relative to the susceptible strain. Detoxification-related genes including P450s, cuticle protein genes, GSTs, UGTs and molecular chaperone HSP70s were overexpressed in the selected resistant strains, especially in the IMI strain. Five genes were downregulated in all three selected resistant strains, including 2 UDP-glucuronosyltransferase 2B18-like genes (LOC 109030370 and LOC 109032577).ConclusionsTen generations of selection with the three neonicotinoids induced different resistance levels and gene expression profiles, mainly involving cuticle protein and P450 genes, in the three selected resistant whitefly strains. The results provide a reference for research on resistance and cross-resistance against neonicotinoids in B. tabaci.

Highlights

Neonicotinoids are widely applied in the control of the destructive agricultural pest Bemisia tabaci, and resistance against these chemicals has become a common, severe problem in the control of whiteflies
Whiteflies of the MEAM1 cryptic species originating from the susceptible parental strain were selected continuously for 10 generations with imidacloprid, acetamiprid or thiamethoxam
The results demonstrated that different resistance levels developed in the whitefly MEAM1 cryptic species in the order of imidacloprid-selected strain (IMI) > acetamipridselected strain (ACE) > THI

Summary

Introduction

Neonicotinoids are widely applied in the control of the destructive agricultural pest Bemisia tabaci, and resistance against these chemicals has become a common, severe problem in the control of whiteflies. The cotton whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is one of the most invasive and destructive pests worldwide. It has a wide host range including more than 600 species of agriculture and horticulture. With the worldwide use of neonicotinoids, whiteflies, especially the MEAM1 and MED cryptic species, have developed different levels of resistance to these insecticides [19,20,21,22,23,24]. Imidacloprid resistance in whiteflies was first reported in 1996, followed by thiamethoxam and acetamiprid resistance [18, 20, 21, 25]

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