Abstract
The phage λ int gene is transcribed from two different promoters, p i and p l . Transcription from p i results in efficient synthesis of Int protein whereas transcription originating from p l results in poor int expression. The differential expression of Int from these two transcripts is dependent upon a site ( sib) located distal to the int gene [Guameros and Galindo, Virology 95 (1979) 119–126; Guameros et al., Proc. Natl. Acad. Sci. USA 79 (1982) 238–242]. We have examined p i -promoted transcription in the region beyond the int coding sequence. The int mRNA extends to a site designated t i , which is located 277 nucleotides beyond int. Characterization of transcription at t i indicates that t i terminates with 75% efficiency in vitro, and that its efficiency is over 95% in vivo. The region between int and t i contains the regulatory signals needed for phage λ integration and appears to be untranslated. The termination site overlaps with the sib control region that reduces Int synthesis from p l transcripts.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
