Transcription profile analysis identifies marker genes to distinguish salt shock and salt stress after stepwise acclimation in Arabidopsis thaliana and Zea mays

Abstract

Many physiological and molecular responses to salt stress have been investigated after a salt shock. However, salt shock rarely happens in agricultural practice. In the field, salts accumulate gradually due to poor agricultural management. Thus in salinity research, it is more reasonable to investigate plant reaction after stepwise acclimation to salt stress. Previous studies demonstrate that salt shock induces Phase 0, a short-term effect that shows transient water loss and rapid turgor decrease; salt stress after stepwise acclimation avoids Phase 0 effects and induces Phase 1. During Phase 1, plants show maintenance of turgor. In this study, salt shock and stepwise acclimation to salt stress were separated at physiological and transcriptional levels. Four major experiments were conducted: 1) leaf turgor changes were monitored in real time after salt application to separate Phase 0 and Phase 1 effects at the physiological level, 2) RNA-sequence analysis was conducted in Arabidopsis thaliana L. to identify potential marker genes that are involved in plant water relations to distinguish Phase 0 and Phase 1 at transcript level, 3) these selected marker gene candidates were identified in Arabidopsis at different Phase 0 and Phase 1 time points via qRT-PCR, 4) these candidates were further evaluated in Zea mays L. (a model plant for applied research in plant physiology and an important crop plant) via qRT-PCR. In future salinity research, marker genes that are both applicable in Arabidopsis and maize have the potential to differentiate salt shock and stepwise acclimation to salt stress.