Transcription of Insulin-like Growth Factor Receptor in Russian Sturgeon (Acipenser gueldenstaedtii) Ovary during Oogenesis

Gad Degani,Avshalom Hurvitz,S Yom Din

doi:10.13189/ujar.2017.050206

Abstract

In the present study, we examined the insulin-like growth factor receptor 1(IGF-1R) transcription using real-time PCR at different stages of oocytes of Russian sturgeon (Acipenser gueldenstaedtii) at age 8-9 years and weight 25 kg that were grown under aquaculture conditions. Compare to the pre-vitellogenesis stage, the transcription of IGF-1R increased at the vitellogenesis stages (yellow, gray and black). The mRNA of IGF-1R of oocytes in the black stage is significantly higher than in the gray or yellow stages. However, there are no significant differences between the gray or yellow stages of the mRNA level. Based on previous studies and the result of the mRNA of IGF-1R of oocytes during oogenesis, we proposed a model for the effect of GH, IGF-1 and IGF-1R on the oogenesis of Russian sturgeon, which is a very important process for creating caviar.

Highlights

Russian sturgeon (Acipenser gueldenstaedtii) belongs to one of the most ancient groups of osteichthyes
In the pre-vitellogenesis stage, the transcription of IGF-1 receptor (IGF-1R) was lower compared to the vitellogenesis of various stages
MRNA of IGF-1R was found to increase dramatically in the ovary during vitellogenesis compared to the pre-vitellogenesis stages

Summary

Introduction

Russian sturgeon (Acipenser gueldenstaedtii) belongs to one of the most ancient groups of osteichthyes. A. gueldenstaedtii belong to the Osteichthyes class, Acrinopteygii sub-class, Acipenseriformes order and Acipenseridae family (Vlasenko et al.[20]). The distribution of this osteichthyes group is along the coasts of the Atlantic and Pacific Oceans, in the Mediterranean and Black Seas, and in many rivers, lakes and inland seas (Dettlaff et al.[5]). The decline of sturgeon populations in their native habitats, mainly in the Caspian Sea, is due to overfishing for meat and caviar production This has led to the destruction of their spawning grounds and water pollution, as well as bringing about their introduction into aquaculture. Spermatogenesis started after two years, while in females, oogenesis starts at the age six years and reaches maturity at around seven years (Hurvitz et al [7]; Hurvitz et al [8]; Jackson et al [6])

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Conclusion