Abstract
DNA-dependent RNA polymerase isolated from lymphocytes of patients with chronic lymphocytic leukemia (CLL) was used to study the transcription of human DNA and chromatins. The α-amanitin sensitive CLL RNA polymerase responds to fewer binding sites on chromatin than on DNA, but both templates have about the same number of binding sites for Escherichia coli RNA polymerase. Both RNA polymerases synthesize RNA with approximately the same efficiency when human chromatin is used as template suggesting that the proportion of DNA available for transcription is the same for the polymerases. Molecular hybridization studies showed that human polymerase transcripts of purified DNA contain more repetitive sequences than do transcripts of DNA complexed in chromatin, whereas bacterial polymerase transcripts are composed of relatively equal amounts of repetitive sequences from both templates.
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