Abstract
An advantage of using isolated nuclei for the study of transcription is the presence of endogenous RNA polymerases. Moreover, in the nuclei, the native state of the chromatin, including regulatory proteins, is maintained. Transcription in nuclei from various plant sources, e.g., soybean hypocotyls [ 1 ] and tobacco callus [2] has been studied. Using tobacco callus nuclei as an in vitro system we did not find any effect of hormones on transcription [3]. However, one should be aware that nuclei isolated from different tissues of the same plant species may behave differently. Therefore, we have compared the characteristics of nuclei isolated from tobacco stem pith, pith callus and leaves. Clear differences between the properties of the nuclei were found in the amounts of RNA synthesized per Irg DNA, in the contents of free RNA polymerases, and in the stimulation of RNA synthesis by heparin. Exogenous DNA of various origins was transcribed to a different extent by the nuclei studied.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
