Transcription factors involved in the development of Purkinje cell dendrites

Abstract

Event Abstract Back to Event Transcription factors involved in the development of Purkinje cell dendrites Isabelle Dusart1* 1 CNRS UMR7102, UPMC, France Purkinje cells (PCs) in the cerebellum generate a very peculiar dendritic tree: very large, highly branched and planar. During the first two postnatal weeks, mouse PCs undergo a 2-phase remodelling process of their dendrites. The first phase is characterized by the retraction of the primitive dendrite and the formation of multiple small processes arising from the cell body, disappearing with the formation of the mature dendrites. In the second phase, the mature dendrites grow rapidly and branch out.In order to identify TFs involved in the dendritic development of PCs, we analyzed the gene expression profile of a cerebellar compartment enriched in PCs. Comparing the transcriptome at different maturation stages; we found that several TFs were differentially expressed during the two phases of dendritic remodelling. Six of them were validated by quantitative RT-PCR and single cell RT-PCR analyses. Among these factors, Ebf1 and Ebf2 were expressed during the first phase of PC dendritic remodelling and downregulated in the second phase. To investigate the contributions of these factors in dendritic development, we generated lentiviral vectors allowing overexpression in organotypic cultures counteracting their developmental downregulation. We show that overexpression of Ebf2 maintained PCs in a morphologic immature stage inhibiting the retraction of the primitive dendritic tree whereas Ebf1 overexpression in PCs resulted only in minor effects on dendritic development. Our results indicate that Ebf2 plays important role in the dendritic development of PCs. Conference: 3rd Mediterranean Conference of Neuroscience , Alexandria, Egypt, 13 Dec - 16 Dec, 2009. Presentation Type: Oral Presentation Topic: Symposium 11 – Development of the cerebellum: from neurogenesis to axon guidance and dendritic differentiation Citation: Dusart I (2009). Transcription factors involved in the development of Purkinje cell dendrites. Front. Neurosci. Conference Abstract: 3rd Mediterranean Conference of Neuroscience . doi: 10.3389/conf.neuro.01.2009.16.055 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 19 Nov 2009; Published Online: 19 Nov 2009. * Correspondence: Isabelle Dusart, CNRS UMR7102, UPMC, Paris, France, isabelle.dusart@upmc.fr Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Isabelle Dusart Google Isabelle Dusart Google Scholar Isabelle Dusart PubMed Isabelle Dusart Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.