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Abstract
BackgroundRecently, overwhelming evidence supports that long noncoding RNAs (lncRNAs) play crucial roles in the occurrence and progression of tumors. However, the role and mechanism of lncRNA TFAP2A-AS1 in human gastric cancer (GC) remains unclear. Thus, the biological role and regulatory mechanisms of TFAP2A-AS1 in GC were explored.MethodsQuantitative real-time PCR (qPCR) was applied to detect gene expression. Western blot was used to measure protein expression. Cell proliferation and migration were determined by functional assays. Fluorescence in situ hybridization (FISH) assays were performed to determine the subcellular distribution of TFAP2A-AS1 in GC. Mechanism investigations were conducted to explore the downstream genes of TFAP2A-AS1 and the upstream transcription factor of TFAP2A-AS1 in GC cells.ResultsTFAP2A-AS1 inhibits the proliferation and migration of GC cells. In the downstream regulation mechanism, miR-3657 was verified as the downstream gene of TFAP2A-AS1 and NISCH as the target of miR-3657. NISCH also suppresses cell proliferation and migration in GC. In the upstream regulation mechanism, transcription factor KLF15 positively mediates TFAP2A-AS1 to suppress GC cell proliferation and migration.ConclusionKLF15-mediated TFAP2A-AS1 hampers cell proliferation and migration in GC via miR-3657/NISCH axis.
Highlights
Overwhelming evidence supports that long noncoding RNAs play crucial roles in the occurrence and progression of tumors
TFAP2A‐AS1 suppresses Gastric cancer (GC) cell proliferation and migration Firstly, we utilized starBase, finding that TFAP2A-AS1 was upregulated in GC tissues compared with normal tissues (Additional file 1: Fig. S1A)
QPCR was implemented to verify whether TFAP2A-AS1 was unusually expressed in AGS, NUGC4, and MKN74 cells, the common cells for GC researches [11, 12], compared with in GES-1 cells
Summary
Overwhelming evidence supports that long noncoding RNAs (lncRNAs) play crucial roles in the occurrence and progression of tumors. The role and mechanism of lncRNA TFAP2A-AS1 in human gastric cancer (GC) remains unclear. The biological role and regulatory mechanisms of TFAP2A-AS1 in GC were explored. Previous studies have reported the functions of lncRNAs in the occurrence and progression of GC from the aspect of cell proliferation, migration and invasion, etc. SNHG5/ miR-32 axis mediates cell proliferation and migration via targeting KLF4 in GC [8]. TFAP2AAS1 has been reported to be key prognostic lncRNA in clear cell renal cell carcinoma [9] and to inhibit cell proliferation and invasion in breast cancer via miR933/SMAD2 axis [10]. The biological role and underlying mechanism of TFAP2A-AS1 in GC remains to be explored
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