Transcription factor Fli-1 is involved in regulation of dendritic cell and monocyte development (153.33)

Abstract

Abstract The Fli1 gene is a member of the ets family of transcription factors that is expressed in hematopoietic cells. We have reported that Fli-1 plays an important role in the B cell and megakaryocyte development. C57BL/6 (B6) mutant Fli-1 mice (Fli-1ΔCTA) express a truncated Fli-1 protein that lack the C-terminal transactivation domain Fli-1ΔCTA B6 mice had significant decreased follicular B cells, increased marginal zone B cells and decreased platelets compared with wild-type mice. In this report, we examined the role of Fli-1 in dendritic cell and monocyte development using Fli-1ΔCTA B6 mice. Fli-1ΔCTA and wild-type B6 mice were sacrificed at the ages of 8-12 weeks and dendrtic cell and monocyte populations in blood, spleen and bone marrow were analyzed with Flow cytometery using specific antibodies. Fli-1ΔCTA B6 mice had significantly increased conventional dendritic cells and monocytes in blood compared with wild-type control B6 mice. Also dendritic cell population in the spleen and the common dendtitic cell precursor population in bone marrow are significantly increased compared to wild-type controls. Our results demonstrate that the Fli1 plays an important role in myeloid cell development and the CTA domain in Fli-1 protein is negatively regulates the myeloid cell development. Further molecular mechanisms regarding effects of Fli-1 on myeloid cell differentiation are under investigation.