Abstract
Transcription factor 21 (TCF21) is one of the essential transcription factors in kidney development. To elucidate its influence on glomerular disease, we have investigated TCF21 expression in human and rat kidney tissue, and its urinary concentration. Immunohistological analysis suggested the highest TCF21 expression in nephrotic syndrome along with the urinary protein level. Urinary TCF21 concentration in human showed a positive correlation with its podocyte expression level. In nephrotic rat models, TCF21 expression in podocytes increased along with the severity of nephrotic syndrome. Next, in vitro experiments using Tcf21-expressing murine podocyte cell line, we could observe some Tcf21-dependent effects, related with actin cytoskeleton dysregulation and apoptosis. Our study illustrated TCF21 expression changes in vivo and its in vitro-functional significance injured podocytes.
Highlights
Transcription factor 21 (TCF21) is one of the essential transcription factors in kidney development
The stain of the supernatant reacted with antigen peptide was not found in non-glomerular disease (NGD), immunoglobulin A nephropathy (IgAN) and membranous glomerulonephritis (MGN) samples, comparing to the positive stain of the anti-TCF21 antibody usage (Supplementary Fig. 1a–c)
The human normal glomeruli showed that TCF21 was weakly expressed in the nuclei of podocytes localized by specific antigens, such as synaptopodin (SYNPO) and nephrin (NPHS1) (Fig. 1a, b)
Summary
Transcription factor 21 (TCF21) is one of the essential transcription factors in kidney development. To elucidate its influence on glomerular disease, we have investigated TCF21 expression in human and rat kidney tissue, and its urinary concentration. In the later stage in Tcf[21] knockout mice, the foot processes, required for the regulation of proteinuria, becomes aberrant in glomerular podocytes. Tcf[21] knockout adult mice showed the development of FSGS with age, and the DKD models changed for the w orse[11]. Even though these results have been established in mice, Santé et de la Recherche Médicale, Unité Mixte de Recherche S1155, SorbonneUniversité, Paris, France. We clarify the contributions of TCF21 to the regulation of proteinuria by detecting podocyteexpressed TCF21 in various glomerular diseases and evaluating its in- vitro effects
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